产细菌素屎肠球菌SC-Y112的体外益生性及安全性评价  

Evaluation of in Vitro Probiotic and Safety Properties of Enterococcus faecium SC-Y112 Producing Bacteriocin

作　　者：罗强 张明[1] 刘巧 罗璠[1] LUO Qiang;ZHANG Ming;LIU Qiao;LUO Fan(Key Laboratory of Ministry of Education for Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation,College of Animal and Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]西南民族大学畜牧兽医学院,青藏高原动物遗传资源保护与利用教育部重点实验室,四川成都610041

出　　处：《食品科学》2021年第11期154-160,共7页Food Science

基　　金：中央高校基本科研业务费专项资金资助项目(2018NZD11)。

摘　　要：屎肠球菌SC-Y112是一种具有广谱抗菌性质的产细菌素乳酸菌,为更深入了解其菌株性质,本研究通过模拟胃肠液耐受实验、胆盐耐受实验、抗氧化活性测定、溶血活性测定、明胶液化实验、有害代谢物质检测、耐药性评价及肠球菌毒力基因检测对屎肠球菌SC-Y112的体外益生性质与安全性质进行分析评价。结果表明:屎肠球菌SC-Y112对模拟胃肠液耐受性强,在模拟胃液培养3 h存活率达73.66%,在0.3 g/100 mL的胆盐质量浓度下培养3 h后存活率达56.38%,其发酵24 h上清液总抗氧化能力达35.21 U/mL;此外,屎肠球菌SC-Y112不产生物胺、吲哚以及偶氮还原酶等有害代谢物质,无溶血现象,不溶解明胶,不携带常见肠球菌毒力基因,且对万古霉素敏感。研究结果可为菌株的进一步开发与应用提供科学依据。Enterococcus faecium SC-Y112 is a bacteriocin-producing lactic acid bacterium with broad-spectrum antibacterial properties.In order to better understand it,the in vitro prebiotic and safety properties of Enterococcus faecium SC-Y112 were explored through simulated gastrointestinal fluid tolerance,bile salt tolerance,antioxidant activity,hemolytic activity,gelatin liquefaction and harmful metabolite tests,as well as the evaluation of drug resistance and the virulence gene analysis of Enterococcus.The results showed that SC-Y112 had a strong tolerance to artificial gastrointestinal juice.The survival rate after 3 hours of exposure to it was 73.66%compared to 56.38%after 3 hours of exposure to 0.3 g/100 mL bile salt,and the total antioxidant activity of the 24 h fermentation supernatant was 35.21 U/mL.In addition,this strain could not produce biological amines,indoles,azoreductase or other harmful substances,caused no hemolysis,did not dissolve gelatin,did not carry the virulence genes of Enterococcus,and was sensitive to vancomycin.These results provide a scientific basis for further development and application of the strain.

关 键 词：屎肠球菌 益生性质 安全性质 毒力基因 

分 类 号：TS201.3[轻工技术与工程—食品科学]