浙江省某地海产品中副溶血性弧菌污染及耐药性分析  

Contamination and antimicrobial resistance of Vibrio parahaemolyticus isolated from seafood in a place of Zhejiang Province

作　　者：毛争争 陈欢[1] 郑琼[1] 贺力 陆欣[1] MAO Zheng-zheng;CHEN Huan;ZHENG Qiong;HE Li;LU Xin(Jiaojiang Center for Disease Control and Prevention,Taizhou,Zhejiang 318000,China)

机构地区：[1]台州市椒江区疾病预防控制中心,浙江台州318000

出　　处：《上海预防医学》2021年第6期500-502,513,共4页Shanghai Journal of Preventive Medicine

基　　金：浙江省台州市椒江区科技计划项目(182038)。

摘　　要：[目的]了解浙江省某区市售海产品中副溶血性弧菌污染状况及耐药性,为防控副溶血性弧菌引起的食源性疾病提供基础数据。[方法]根据GB 4789.7-2013标准对海产品中副溶血性弧菌进行分离鉴定,利用实时荧光聚合酶链反应(PCR)法鉴定毒力基因,采用K-B法进行药敏试验。[结果]从210份样品中检出副溶血性弧菌阳性样本55份,检出率为26.19%,不同海产品种类检出率差异较大,腹足类和双壳类污染较为严重。分离所得的55株副溶血性弧菌均不含毒力基因tdh和trh,所有菌株均检出2种特异性基因tlh和toxR。大部分菌株对氨苄西林和阿莫西林耐药,耐药率均为96.36%。所有菌株对氨苄西林/舒巴坦、四环素、环丙沙星、左氧氟沙星、氧氟沙星、氯霉素完全敏感。[结论]市售海产品普遍存在副溶血性弧菌污染,分离菌株存在一定程度的耐药情况,应加强海产品副溶血性弧菌污染状况监测,规范养殖和临床抗生素使用。[Objective]To understand the contamination and antimicrobial resistance of Vibrio parahaemolyticus in seafood sold in a place of Zhejiang Province,and to provide basic data for the prevention and control of food-borne diseases caused by V.parahaemolyticus.[Methods]V.parahaemolyticus was isolated and identified from seafood according to GB 4789.7—2013 method.The virulence genes were identified by real-time fluorescent PCR,and the antimicrobial sensitivity test was performed by Kirby-Bauer method.[Results]V.parahaemolyticus was isolated from 55 of 210 seafood samples.The detection rate was 26.19%,and the detection rates of different species were quite different.None of the 55 isolates contained virulence genes tdh and trh,while all isolates have species-specific genes tlh and toxR.Most isolates were resistant to ampicillin and amoxicillin,with the same antimicrobial resistance rate of 96.36%.All isolates were fully sensitive to ampicillin/sulbactam,tetracycline,ciprofloxacin,levofloxacin,loxacin and chloramphenicol.[Conclusion]V.parahaemolyticus contamination is common in sold seafood.The antimicrobial resistance of the isolates has reached a certain level.Monitoring of the pollution status of V.parahaemolyticus in seafood should be strengthened and the aquaculture and clinical use of antibiotics should be standardized.

关 键 词：副溶血性弧菌 海产品 毒力基因 耐药性 

分 类 号：R378.3[医药卫生—病原生物学]