Aspirin Enhances the Protective Effect of Progesterone on Trophoblast Cell from Oxidative Stress and Apoptosis  

作　　者：Jia-Wei Shi Hui-Li Yang Zhen-Zhen Lai Hui-Hui Shen Lu-Yu Ruan Yan Wang Xue-Min Qiu Feng Xie Ming-Qing Li 

机构地区：[1]Laboratory for Reproductive Immunology,Institute of Obstetrics and Gynecology,Hospital of Obstetrics and Gynecology,Shanghai Medical School,Fudan University,Shanghai 200080,China [2]Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases,Shanghai,200080,China

出　　处：《Reproductive and Developmental Medicine》2021年第1期1-8,共8页生殖与发育医学（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China(No.31970798,31671200,82072872);the Innovation-oriented Science and Technology Grant from NPFPC Key Laboratory of Reproduction Regulation(CX2017-2);the Program for Zhouxue of Fudan University(JIF157602);the Support Project for Original Personalized Research of Fudan University.

摘　　要：Objective:Clinically,low-dose aspirin and progesterone are frequently used to prevent pregnancy loss.We investigated the effect of these drugs on the biological behavior of human extravillous trophoblasts in vitro.Methods:HTR-8/SVneo cells were cultured in vitro and treated with different concentrations of aspirin and progesterone.The proliferation,invasion,and apoptosis of HTR-8/SVneo cells were assessed using a cell counting Kit-8 assay,Matrigel Transwell assay,and Hoechst staining,respectively.Reverse transcriptase polymerase chain reaction was used to verify the expression of related genes.Reactive oxygen species(ROS)levels were detected using the 2,7-dichlorofluorescin diacetate assay.Results:Low-dose aspirin alone,progesterone alone,or aspirin plus progesterone upregulated the proliferation and invasion and decreased the apoptosis of HTR-8/SVneo cells.Moreover,the expression of marker of proliferation Ki-67(MKI67),matrix metalloproteinases 2(MMP2),and MMP9 was increased.In addition,low-dose aspirin plus progesterone exerted stronger anti-apoptosis effects than low-dose aspirin and progesterone alone.Interestingly,aspirin upregulated the expression of progesterone receptor(PGR).Treatment with hydrogen peroxide(H_(2)O_(2))promoted ROS production in HTR-8/SVneo cells;however,low-dose aspirin plus progesterone significantly restricted H_(2)O_(2)-mediated ROS production and apoptosis in HTR-8/SVneo cells.Conclusions:These data suggest that low-dose aspirin and progesterone promote proliferation and invasion and cooperatively reduce oxidative stress and apoptosis in trophoblasts in vitro.These results may provide an experimental basis for the combined application of aspirin and progesterone to prevent unexplained recurrent spontaneous miscarriage,especially in patients with trophoblast dysfunction.

关 键 词：APOPTOSIS ASPIRIN Invasion MISCARRIAGE PROGESTERONE Progesterone Receptor Proliferation Reactive Oxygen Species TROPHOBLASTS 

分 类 号：R965[医药卫生—药理学]