转人溶菌酶奶牛牛乳蛋白理化性质的研究  

Study on the physicochemical property of transferred human lysozyme cow milk protein

作　　者：程静然 宋荣荣 戴蕴平[2] 赵春江[1] CHENG Jingran;SONG Rongrong;DAI Yunping;ZHAO Chunjiang(College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;College of Biology,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学动物科学技术学院,北京100193 [2]中国农业大学生物学院,北京100193

出　　处：《黑龙江畜牧兽医》2021年第11期12-16,149,150,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：“抗病转基因牛新品种培育”重大专项(2016ZX08007-003)。

摘　　要：为了探究转人溶菌酶奶牛牛乳的安全性,试验以转人溶菌酶基因奶牛牛乳为研究对象、非转基因奶牛牛乳为对照,对转基因组和非转基因组奶牛牛乳进行乳成分差异比较,并分析了重组人溶菌酶的分子结构。结果表明:重组人溶菌酶在转人溶菌酶奶牛乳腺中实现了高表达,转基因组和非转基因组的乳清差异蛋白共有147种,包括抗菌和抑菌等相关蛋白;纯化后的重组人溶菌酶肽指纹与天然人溶菌酶相符,重组人溶菌酶的分子质量为14 691.859 u,两种酶分子质量相近;N末端序列相同,均为KVFERCELARTLKRL;均有4种类型的二级结构,α-螺旋、β-转角、β-折叠、无规则卷曲的百分比含量分别为44.62%、14.62%、8.46%和32.30%。说明转基因因素没有引起转基因牛乳主要成分发生较大变化,重组人溶菌酶和天然人溶菌酶基本一致,初步证明转人溶菌酶奶牛牛乳在蛋白理化性质方面是安全的。In order to study the safety of transferred human lysozyme cow milk, human lysozyme cow milk gene was used as study subject, and transgenic cow milk was used as control.The differences of milk composition between transgenic and non-transgenic cows were compared, and the molecular structure of the recombinant human lysozyme were analyzed. The results showed that the recombinant human lysozyme was highly expressed in the transgenic cows, and there were 147 different proteins between two groups including antibacterial and bacteriostatic proteins. The fingerprint of the purified recombinant human lysozyme peptide was consistent with natural human lysozyme. The molecular weight of recombinant human lysozyme was 14 691.859 u, and the molecular weight of the two enzymes was similar, and they had the same N-terminal sequence and were all KVFERCELARTLKRL. They all had four types of secondary structures, and the percentages of α-helix, β-corner, β-fold and random coil are 44.62%, 14.62%, 8.46% and 32.30%, respectively. It indicated that genetically modified factors did not cause great changes in the main components of transgenic milk, and the recombinant human lysozyme was basically consistent with natural human lysozyme, which preliminarily proved that milk of transgenic cloned cows expressing human lysozyme was safe in physicochemical property.

关 键 词：转基因牛 重组人溶菌酶 牛乳 蛋白质 理化性质 

分 类 号：S823[农业科学—畜牧学] Q51[农业科学—畜牧兽医]