QuEChERS-高效液相色谱-柱后光化学衍生法测定粮谷类食品中黄曲霉毒素  

Determination of aflatoxin in cereal food by QuEChERS-high performance liquid chromatography with post-column photochemical derivatization

作　　者：姚誉阳[1] 顾妍丽[1] 吴宇伉 朱鹏飞[1] 孟元华[1,2] 朱慧敏 YAO Yu-Yang;GU Yan-Li;WU Yu-Kang;ZHU Peng-Fei;MENG Yuan-Hua;ZHU Hui-Min(Wuxi Centre for Disease Control and Prevetion,Wuxi 214023,China;Public Health Research Centre,Jiangnan University,Wuxi 214122,China)

机构地区：[1]无锡市疾病预防控制中心,无锡214023 [2]江南大学公共卫生研究中心,无锡214122

出　　处：《食品安全质量检测学报》2021年第9期3497-3502,共6页Journal of Food Safety and Quality

基　　金：无锡市“科教强卫工程”项目(CXPT(SYS)002);江南大学公共卫生研究中心项目(JUPH201818)。

摘　　要：目的建立QuEChERS-高效液相色谱-柱后光化学衍生法测定粮谷类食品中黄曲霉毒素B_(1)、B_(2)、G_(1)、G2的分析方法。方法样品经过1%甲酸-乙腈提取,MgSO_(4)+C_(18)+PSA净化,高效液相色谱-柱后光化学衍生化法检测。结果黄曲霉毒素B_(1)、G_(1)和黄曲霉毒素B_(2)、G2分别在0.5~20、0.125~5μg/L范围内线性关系良好,相关系数均大于0.999,方法检出限为0.1~0.3μg/kg,定量限为0.3~1.0μg/kg,加标回收率为86.4%~97.8%,相对标准偏差(relative standard deviation,RSD)为2.9%~6.3%。结论该方法前处理简便,回收率稳定,测定结果准确,成本低廉,可用于粮谷类食品中黄曲霉毒素的大批量检测。Objective To establish a method for the determination of aflatoxin B_(1),B_(2),G_(1),G2 in cereal food by QuEChERS-high performance liquid chromatography with post-column photochemical derivatization.Methods The sample was extracted with 1%formic acid-acetonitrile,purified by MgSO_(4)+C_(18)+PSA and detected by high performance liquid chromatography with post-column photochemical derivatization.Results Aflatoxin B_(1),G_(1)and Aflatoxin B_(2),G2 were good in linear relationship between 0.5-20 and 0.125-5μg/L separately,and the relative coefficients were more than 0.999.The limits of detection(LOD)of the method were 0.1-0.3μg/kg and the limits of quantification(LOQ)were 0.3-1.0μg/kg.The recoveries of the method were 86.4%-97.8%,and the relative standard deviations were 2.9%-6.3%.Conclusion This method has simple pretreatment,stable recovery,accurate determination and low cost,and can be applied for the mass detection of aflatoxin in cereal food.

关 键 词：QuEChERS提取 高效液相色谱法 柱后光化学衍生法 粮谷类食品 黄曲霉毒素 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程] O657[轻工技术与工程—食品科学与工程]