分型方法在单核细胞增多性李斯特菌监测溯源中的应用研究进展  

Research progress on the application of typing methods for monitoring and source tracking of Listeria monocytogenes

作　　者：杜冬冬 钱晶 王静[1] 王国红[1] 罗瑞峰[1] 殷月兰[2] 黄新[3] 张星星[3] 康立超[1] DU Dong-Dong;QIAN Jing;WANG Jing;WANG Guo-Hong;LUO Rui-Feng;YIN Yue-Lan;HUANG Xin;ZHANG Xing-Xing;KANG Li-Chao(Analysis and Testing Center,Xinjiang Academy of Agriculture and Reclamation Science,Xinjiang Production and Construction Corps Institute for Food Control,Shihezi 832000,China;Jiangsu Key Laboratory of Zoonosi,Yangzhou 225000,China;Key Laboratory of Sheep Breed and Biotechnology of Xinjiang Production and Construction Corps,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,China)

机构地区：[1]新疆农垦科学院分析测试中心,新疆生产建设兵团食品检验所,石河子832000 [2]江苏省人兽共患病学重点实验室,扬州225000 [3]新疆农垦科学院省部共建绵羊遗传改良与健康养殖国家重点实验室,石河子832000

出　　处：《食品安全质量检测学报》2021年第9期3813-3819,共7页Journal of Food Safety and Quality

基　　金：江苏省人兽共患病学重点实验室资助项目(R1901);兵团生产建设兵团重点领域科技攻关计划项目(2019AB029);兵团国际科技合作计划项目(2019BC004)。

摘　　要：单核细胞增多性李斯特菌(Listeriamonocytogenes,LM)简称单增李斯特菌,是引起人和动物李斯特菌病的重要食源性病原菌,具有较高的病死率,严重危害人类公共卫生安全和畜牧业发展。快速准确地对LM鉴定和溯源,对有效控制李斯特菌病的暴发和蔓延发挥重要作用。建立高效快捷的分型方法是LM溯源的关键。目前LM分型方法主要分为表型分型和分子分型,每种分型方法各有优劣,适用不同的流行病学调查。本文综述了LM分型方法的研究进展,以期为开展LM的暴发确认和溯源检测方法的选择提供参考依据。Listeria monocytogenes(LM)is described as an important foodborne pathogen that can cause humanand animals listeriosis,with the characteristics of high mortality.LM is seriously threatens the development of animal husbandry,human health and safety.It plays an important role in effectively controlling the foodborne disease outbreak and spread of listeriosis by rapid and accurate identification of LM pollution sources.Simple and efficient tracing techniques and typing methods are key to trace the source of LM.Currently,typing methods of LM include phenotypic typing and molecular typing.These typing methods have their own advantages and limitations,and have different applications on different epidemiological investigating.This paper reviewed the research progress of LM typing,in order to provide reference for the outbreak confirmation of LM and the selection of traceability detection methods.

关 键 词：单核细胞增多性李斯特菌 表型分型方法 分子分型方法 溯源方法 

分 类 号：TS201.3[轻工技术与工程—食品科学]