微孔侧流免疫层析法检测农产品中2, 4-二氯苯氧乙酸残留  

Detection of residual 2,4-dichlorophenoxyacetic acid in agriculture products by microwell lateral flow immunochromatography assay

作　　者：华彦涛 刘波[1] 赵炫 尹凯丹[1] 马楠楠 袁利鹏[1] HUA Yantao;LIU Bo;ZHAO Xuan;YIN Kaidan;MA Nannan;YUAN Lipeng(Guangdong AIB Polytechnic College,Guangzhou 510665,China;Guangzhou Wanlian Biotechnology Company,Guangzhou 510663,China)

机构地区：[1]广东农工商职业技术学院,广东广州510665 [2]广东万联生物科技有限公司,广东广州510663

出　　处：《食品与发酵工业》2021年第12期244-249,共6页Food and Fermentation Industries

基　　金：广东省教育厅工程技术研究中心(2019GGCZX010);广东省教育厅2016高等职业教育专业领军人才项目;全国高等职业技术教育研究会教改课题(GZYLX2015011);广东省教育厅教学成果奖培育项目(201572991)。

摘　　要：该研究采用活泼酯法制备了人工抗原,通过动物免疫获得了针对2,4-二氯苯氧乙酸的特异性抗体。通过试验条件优化,建立了微孔侧流免疫层析方法,用于水果和蔬菜中2,4-二氯苯氧乙酸残留的快速检测。该方法的检测限:2,4-二氯苯氧乙酸为0.05μg/mL,4-氯苯氧乙酸为0.2μg/mL,2,4,5-三氯苯氧乙酸为0.5μg/mL。实际样品的检测中,其结果与HPLC的结果相一致率为100%。检测方法前处理简单、快速灵敏,且能够同时检测2,4-二氯苯氧乙酸及其结构类似物,适用于水果蔬菜中2,4-二氯苯氧乙酸残留的快速筛选。The coating antigen and immunogen of the 2,4-dichlorophenoxyacetic acid(2,4-D)were prepared by the active ester method,respectively.The polyclonal antibody was obtained by immunizing rabbits,which could simultaneously recognize 2,4-dichlorophenoxyacetic acid.A microwell lateral flow immunochromatographic assay(mwLFIA)was developed for the fast detection of 2,4-D in agriculture products.The LOD of mwLFIA for 2,4-dichlorophenoxyacetic acid,4-Chlorophenoxyacetic acid and 2,4,5-trichlorophenoxyacetic acid were 0.05μg/mL,0.2μg/mL and 0.5μg/mL respectively.And it was accordance with HPLC results.The developed mwLFIA strip was suitable for the rapid detection of 2,4-dichlorophenoxyacetic acid and structural analogs in agriculture products.

关 键 词：2 4-二氯苯氧乙酸 半抗原 多克隆抗体 微孔侧流免疫层析 快速检测 

分 类 号：TS207.53[轻工技术与工程—食品科学]