甲状腺乳头状癌预后标志物的研究  

Research on FN1,SMAD9,TGF-β1 and SPARC as prognostic markers of thyroid papillary carcinoma

作　　者：陈帅[1] 罗锦琳 罗霁 张超杰[1] 邹联洪 范培芝[1] Chen Shuai;Luo Jin-ling;Luo Ji;Zhang Chao-jie;Zou Lian-hong;Fan Pei-zhi(The First Affiliated Hospital of Hunan Normal University/Hunan Provincial People's Hospital,Changsha 410002,China;Xiangya Changde Hospital,Changde 415003,China;Hunan Provincial Key Laboratory of Emergency and Critical Care Metaborutmics,Changsha 410002,China)

机构地区：[1]湖南师范大学第一附属医院/湖南省人民医院,长沙410002 [2]湘雅常德医院,常德415003 [3]急危重症代谢组学湖南省重点实验室,长沙410002

出　　处：《湖南师范大学学报（医学版）》2021年第2期4-7,共4页Journal of Hunan Normal University(Medical Sciences)

基　　金：国家自然科学基金(81602351);湖南省临床医疗技术创新引导项目(2018SK50720);湖南省自然科学基金项目(2017JJ3171);湖南省卫健委重点项目(A2017003);长沙市科技计划项目(kq1701045/Kq1701053)。

摘　　要：目的:运用微阵列和生物信息学分析来揭示与甲状腺乳头状癌(PTC)预后不良有关的基因。方法:选取2016年9月~2018年9月期间入住湖南省人民医院乳甲外科并确诊为甲状腺乳头状癌患者的癌组织和对应癌旁组织对进行基因表达谱分析和差异表达基因的筛选;采用DAVID软件对差异基因行GO功能富集分析和信号通路富集(KEGG)分析,采用STRING数据库对筛选出的差异基因进行蛋白互作网络分析;利用TCGA数据库平台,采用Kaplan-Meier分析方法进行生存分析。结果:甲状腺乳头状癌组织中共筛选出差异表达的基因364个,差异表达的LncRNA 228个。GO分析结果显示:甲状腺乳头状癌差异基因在生物过程中主要富集于"负增长调节"。在分子功能中显著富集于"蛋白酶结合"。在细胞组成中,差异基因主要富集在"细胞外小体"。KEGG信号通路分析提示矿物吸收、胃酸分泌及TGF-β信号通路与甲状腺乳头状癌相关。PPI网络显示GAC前15位核心基因。基于TCGA数据库的生存分析发现FN1、SMAD9与甲状腺乳头状癌的预后负相关,SPARC、TGFβR1的表达与PTC预后正相关。结论:FN1、SMAD9、SPARC、TGFβR1可能作为PTC靶向治疗的潜在分子靶点。Objective To investigate the genes associated with poor prognosis in thyroid papillary carcinoma(PTC)Using exert microarray and bioinformatics analysis technology.Methods The gene profiles of 3 pairs of thyroid papillary carcinoma tissue and corresponding adjacent tissue diagnosed with hyroid papillary carcinoma at Hunan Provincial People’s Hospital from September 2016 to September 2018 were detected using gene expression spectrum chips.The differential expression genes were analyzed using t test.The DAVID software is used to analyze GO functional enrichment analysis and signal pathway enrichment(KEGG)analysis,and the protein interaction network analysis was carried out by STRING database.Survival analysis was carried out by kaplan-Meier analysis with TCGA database.Results Compared with adjacent tissues,364 differentially expressed genes and 228 differentially expressed LncRNA were screened out in PTC.The results of GO analysis showed that the differential genes of PTC were mainly enriched in"negative growth regulation"in the biological processes.In the molecular function,it is significantly enriched in"protease binding".In cell composition,the differential genes are mainly enriched in"extracellular bodies".KEGG signal pathway analysis displayed that mineral absorption,gastric acid secretion and TGF-β signal pathway were associated with PTC.PPI network showed that the top 15 core genes.The survival analysis,which based on TCGA database,showed that the prognosis of PTC was negatively correlated with FN1 and SMAD9,positively correlated with SPARC and TGFβR1.Conclusion FN1,SMAD9,SPARC and TGFβR1 may be potential molecular targets for targeted therapy of PTC.

关 键 词：甲状腺乳头状癌 FN1 TGFβR1 SAMAD9 SPARC 

分 类 号：R736.1[医药卫生—肿瘤]