小麦和大麦麸质蛋白的提取方法比较及SDS-PAGE分析  

Comparison of Extraction Methods of Wheat and Barley Gluten Protein and SDS-PAGE Analysis

作　　者：汪佳佳 胡慧敏 潘雪峰 陈霞 庄昕波 张涛 陈银基[1] Wang Jiajia;Hu Huimin;Pan Xuefeng;Chen Xia;Zhuang Xinbo;Zhang Tao;Chen Yinji(School of Food Science and Engineering,Nanjing University of Finance and Economics,Nanjing,Jiangsu 210023;Anhui Yunyan Food Technology Co.,Ltd.,Xuancheng,Anhui 341881;Agricultural and Rural Bureau of Gaochun District,Nanjing,Jiangsu 211300)

机构地区：[1]南京财经大学食品科学与工程学院,江苏南京210023 [2]安徽云燕食品科技有限公司,安徽宣城341881 [3]南京市高淳区农业农村局,江苏南京211300

出　　处：《粮食科技与经济》2021年第2期91-95,共5页Food Science And Technology And Economy

基　　金：国家自然科学基金面上项目(31871822)。

摘　　要：以小麦和大麦为材料,分别采用混合物提取法、乙醇提取法、异丙醇结合DTT提取法、异丙醇结合DTT两步提取法、水-氯化钠-乙醇三步提取法及水-氯化钠-乙醇水浴提取法对小麦和大麦进行麸质蛋白提取,比较不同方法提取液中蛋白质的质量浓度,并进行SDS-PAGE分析比较其蛋白组成的差异。结果显示,除水-氯化钠-乙醇提取法不能完整提取小麦麸质蛋白,其余几种方法均成功提取麸质蛋白。混合物提取法、60%乙醇提取法、异丙醇结合DTT提取法、70%乙醇提取法从大麦中提取的麸质蛋白质量浓度较高。混合物提取法提取液中SDS-PAGE条带数最多,并且可以提取高分子量麦谷蛋白。Gluten of wheat and barley were extracted by nine extraction methods,SDS-PAGE was applied to separate and compare the extraction results.The results revealed that the extraction method of H2O-NaCl-ethanol could not completely extract gluten proteins,While other methods were successful.Mixture extraction,60%ethanol extraction,Isopropanol/DTT extraction and 70%ethanol extraction obtained higher gluten content in barley.The mixture extraction method extracts the largest number of SDS-PAGE bands in grains and can extract high-molecular-weight glutenin.

关 键 词：小麦 大麦 麸质蛋白 提取 SDS-PAGE 

分 类 号：TS201.2[轻工技术与工程—食品科学]