转氨酶固定化及其连续流催化合成(R)-1-(4-溴苯基)乙胺  

Immobilization ofω-transaminase and its catalytic synthesis of(R)-1-(4-bromophenyl)ethylamine in continuous flow

作　　者：张加强 陆群[1] 赵昱 ZHANG Jiaqiang;LU Qun;ZHAO Yu(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China;Engineering Laboratory ofDevelopment and Utilization of Medicinal Insects and Arachnids Resources in Yunnan Province,Dali 671000,China)

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031 [2]云南省药用昆虫及蛛形类资源开发利用工程实验室,云南大理671000

出　　处：《发酵科技通讯》2021年第2期109-113,共5页Bulletin of Fermentation Science and Technology

基　　金：国家发改委中国西南药用昆虫资源开发协同创新研究基金(VQ99QT1113Y17001)。

摘　　要：构建连续流反应系统以不对称合成(R)-1-(4-溴苯基)乙胺。通过优化转氨酶ATA-117固定于环氧基树脂载体ES-103B的固定化条件,确定固定化酶ATA117@ES103B的最适反应条件,并对所建连续流反应器系统的工艺条件进行优化。20 mmol/L对溴苯乙酮在7 min的停留时间内转化率即可达到93%,时空产率为789.85 g/(L·d)。研究为生物法催化生产(R)-1-(4-溴苯基)乙胺工业化提供可靠的参考。It was constructed for a continuous flow reaction system to synthesize(R)-1-(4-bromophenyl)ethylamine asymmetrically.By optimizing the immobilization conditions of transaminase ATA-117 immobilized on the epoxy resin carrier ES-103B,the optimal reaction conditions of immobilized enzyme ATA117@ES103B were determined,and the process conditions of the continuous flow reactor system were optimized.In the constructed continuous flow reaction system,the conversion rate of 20 mmol/L p-bromoacetophenone can reach 93%in 7 min contact time,and the calculated space-time yield is 789.85 g/(L·d).This method provides a reliable reference for the production of(R)-1-(4-bromophenyl)ethylamine by industrialized bio-catalysis.

关 键 词：转氨酶 环氧基树脂 固定化酶 (R)-1-(4-溴苯基)乙胺 连续流动 

分 类 号：Q814.2[生物学—生物工程]