Chemical modifications of proteins and their applications in metalloenzyme studies  

作　　者：Nathchar Naowarojna Ronghai Cheng Juan Lopez Christina Wong Lu Qiao Pinghua Liu 

机构地区：[1]Department of Chemistry,Boston University,Boston,MA,02215,United States

出　　处：《Synthetic and Systems Biotechnology》2021年第1期32-49,共18页合成和系统生物技术（英文）

基　　金：This research was partially supported by National Science Foundation(CHE-2004109 to P.L.)。

摘　　要：Protein chemical modifications are important tools for elucidating chemical and biological functions of proteins.Several strategies have been developed to implement these modifications,including enzymatic tailoring reactions,unnatural amino acid incorporation using the expanded genetic codes,and recognition-driven transformations.These technologies have been applied in metalloenzyme studies,specifically in dissecting their mechanisms,improving their enzymatic activities,and creating artificial enzymes with non-natural activities.Herein,we summarize some of the recent efforts in these areas with an emphasis on a few metalloenzyme case studies.

关 键 词：METALLOENZYMES Biorthogonal reactions Protein engineering Non-canonical amino acids BIOCATALYSIS 

分 类 号：O62[理学—有机化学]