Tuning osteoporotic macrophage responses to favour regeneration by Cu-bearing titanium alloy in Porphyromonas gingivalis lipopolysaccharide-induced microenvironments  

作　　者：Xiongcheng Xu Yanjin Lu Ling Zhou Mengjiao He Jin Zhuo Quan Zhong Kai Luo Jinxin Lin 

机构地区：[1]Fujian Key Laboratory of 0ral Diseases&Fujian Provincial Engineering Research Center of Oral Biomaterial&Stomatological Key laboratory of Fujian College and University,School and Hospital of Stomatology,Fujian Medical University,Fuzhou 350002,China [2]Institute of Stomatology&Laboratory of Oral Tissue Engineering,School and Hospital of Stomatology,Fujian Medical University,Fuzhou 350002,China [3]Key Laboratory of Optoelectronic Materials Chemistry and Physics,Fujian Institute of Research on the Structure of Matter,Chinese Academy of Sciences,Fuzhou 350002,China [4]University of Chinese Academy of Sciences,Beijig 100049,China [5]Department of Stomatology,Fujian Provincial Governmental Hospital&Fujian Health College Afilited Hospital,Fuzhou 350003,China

出　　处：《Regenerative Biomaterials》2021年第1期102-111,共10页再生生物材料（英文版）

基　　金：This study was supported by National Natural Science Foundation of China(Grant No.81870766,51801198);the Science Foundation of Fujian Province(Grant No.2017J01805);Joint Funds for the Innovation of Sciences and Technology,Fujian Province(Grant No.2016Y9023);Startup Fund for scientific research,Fujian Medical University(Grant No.2019QH2041).

摘　　要：Guided bone regeneration in inflammatory microenvironments of osteoporotic patients with large alve-olar bone defects remains a great challenge.Macrophages are necessary for alveolar bone regeneration via their polarization and paracrine actions.Our previous studies showed that Cu-bearing Ti6AI4V alloys are capable of regulating macrophage responses.When considering the complexity of oral microenvir-onments,the influences of Cu-bearing Ti6AI4V alloys on osteoporotic macrophages in infectious microenvironments are worthy of further investigations.In this study,we fabricated Ti6AI4V-Cu alloy by selective laser melting technology and used Porphyromonas gingivalis lipopolysaccharide(P.g-LPS)to imitate oral pathogenic bacterial infections.Then,we evaluated the impacts of T6AI4V-Cu on osteoporotic macrophages in infectious microenvironments.Our results indicated that Ti6AI4V-Cu not only inhibited the P.g-LPS-induced M1 polarization and pro-inflammatory cytokine production of osteoporotic macrophages but also shifted polarization towards the pro-regenerative M2 phenotype and remarkably promoted antinflammatory cytokine release.In addition,T6AI4V-Cu effectively promoted the actity of COMMD1 to potentially repress NF-κB-mediated transcription.It is concluded that the Cubearing Ti6AI4V alloy results in ameliorated osteoporotic macrophage responses to create a favourable microenvironment under infectious conditions,which holds promise to develop a GBR-barrier membrane for alveolar bone regeneration of osteoporosis patients.

关 键 词：copper OSTEOPOROSIS MACROPHAGES inflammation 

分 类 号：TG146.23[一般工业技术—材料科学与工程]