响应面法优化多聚原飞燕草素超声波降解工艺及其抗氧化性的研究  

Optimization Technology of Ultrasonic Degradation of Polymeric Prodelphinidin by Response Surface Methodology and Its Antioxidant Activity

作　　者：姜坤 叶焕烽 叶秦轩 杨海花 JIANG Kun;YE Huanfeng;YE Qinxuan;YANG Haihua(College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《食品工业科技》2021年第13期221-229,共9页Science and Technology of Food Industry

基　　金：国家自然科学基金青年科学基金项目(31501472);中国博士后基金面上项(2015M582710);中国博士后科学基金第九批特别资助项目(2016T90951)。

摘　　要：研究从杨梅叶中提取制备多聚原飞燕草素,并采用HPLC-DAD-ESIMS等方法对其结构进行了表征。利用单因素实验分析了超声时间、温度等超声波处理条件对多聚体的降解规律。在此基础之上,通过Box-Behnken响应面试验研究了超声波降解多聚原飞燕草素的条件,并采用DPPH·法和ABTS·^(+)法比较了降解前后的抗氧化活性。结果表明制备的多聚原飞燕草素提取率为5.96%,平均聚合度为27.8,其结构组成主要为EGCG。超声波处理不同条件均对多聚体的降解有影响作用,其中主要影响因素为时间、温度、功率与占空比(P<0.05)。通过BoxBehnken响应面试验确定的最优降解条件为超声时间74 min,温度44℃,超声功率361 W,脉冲占空比67%,对应小分子的低聚原花色素得率为17.31%。超声波处理后多聚原飞燕草素相比处理前抗氧化活性显著提高(P<0.01)。In this research,polymeric prodelphinidins(PPs)were extracted and prepared from bayberry leaves and characterized using HPLC-DAD-ESIMS.Then effects of ultrasonic time,temperature,power and duty cycle on PPs were analyzed using one single factor test.On the above bases,the response surface methodology with Box-Behnken design was employed to study the ultrasonic conditions.Furthermore,the antioxidant capacity of PPs were determined before and after ultrasonic treatment by DPPH·and ABTS·^(+)methods.The results showed that yield of PPs from bayberry leaves was 5.96%(dry weight)with an average degree of polymerization of 27.8 and with a main structural component of EGCG.Ultrasonic treatments with different conditions had different influence on PPs degradation.Factors of ultrasonic time,temperature,power and duty cycle had significant effects(P<0.05).The optimized ultrasonic conditions were as follows:Ultrasonic time74 min,temperature 44℃,ultrasonic power 361 W,and duty cycle 67%.Under these conditions,the yield of oligomeric prodelphinidins was 17.31%.After sonication,PPs possessed more significant antioxidant capacity than before(P<0.01).

关 键 词：多聚体原花色素 超声降解 响应面法 抗氧化 

分 类 号：TS201.1[轻工技术与工程—食品科学]