黄芪对2型糖尿病大鼠碱性磷酸酶与炎症反应的影响  

Effect of Astragalus on Intestinal Alkaline Phosphatase and Inflammatory Response in Type 2 Diabetic Rats

作　　者：王语聪 谢智鑫 张学艳[3] 杨秋萍 韩建春[1,2] WANG Yucong;XIE Zhixin;ZHANG Xueyan;YANG Qiuping;HAN Jianchun(College of Food Science,Northeast Agricultural University,Harbin 150030,China;Heilongjiang Green Food Science Research Institute,Harbin 150028,China;Northeast Agricultural University Hospital,Harbin 150030,China)

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]黑龙江省绿色食品科学研究院,黑龙江哈尔滨150028 [3]东北农业大学医院,黑龙江哈尔滨150030

出　　处：《食品工业科技》2021年第13期351-357,共7页Science and Technology of Food Industry

基　　金：东北农业大学校内基金(51800312)。

摘　　要：目的:探究黄芪对2型糖尿病大鼠碱性磷酸酶、细菌内毒素和血清中炎症因子的影响。方法:采用高糖高脂饮食+链脲佐菌素腹腔注射建立2型糖尿病模型,连续给予大鼠灌胃黄芪水溶液(0.25、0.5 g/kg),测定大鼠体质量及血糖变化、粪便中肠道碱性磷酸酶(IAP)的活力及细菌内毒素(LPS)的浓度变化并用MSD多因子方法检测血清中炎症因子(白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、γ干扰素(IFN-γ)、白细胞介素-1(IL-1β)、白介素-4(IL-4)、白介素-5(IL-5)、白介素-10(IL-10)、白介素-13(IL-13)、生长调节致癌基因α(KC/GRO))浓度变化的分析。结果:与模型组相比,黄芪剂量组空腹血糖浓度显著降低(P<0.05),IAP活力显著增加(P<0.05),LPS浓度显著降低(P<0.05),且血清中促炎因子IL-6、TNF-α、IFN-γ、IL-1β的浓度显著减少(P<0.05),而抗炎因子IL-4、IL-5、IL-10、IL-13的浓度显著增加(P<0.05),趋化因子KC/GRO的浓度显著下降(P<0.05)。结论:黄芪可以促进肠碱性磷酸酶活性,并能够有效调节T2DM小鼠血糖及炎症反应。Objective:To explore the effects of Astragalus on alkaline phosphatase,bacterial endotoxin and serum inflammatory factors in type 2 diabetes rats.Methods:A high-sugar and high-fat diet+intraperitoneal injection of streptozotocin was used to establish a type 2 diabetes model.The rats were continuously administered Astragalus aqueous solution(0.25,0.5 g/kg)and measureed the changes in body mass,blood sugar,the activity of intestinal alkaline phosphatase(IAP)in feces and the changes in bacterial endotoxin(LPS)concentration.MSD multi-factor method was used to detect serum inflammatory factors(Interleukin-6(IL-6),Tumor necrosis factor-α(TNF-α),Interferon-γ(IFN-γ),Interleukin-1(IL-1β),Interleukin-4(IL-4),Interleukin-5(IL-5),Interleukin-10(IL-10),interleukin-13(IL-13)growth regulatory oncogeneα(KC/GRO))concentration changes.Results:Compared with the model group,the fasting blood glucose concentration in the Astragalus dose group was significantly reduced(P<0.05),the IAP activity was significantly increased(P<0.05),the LPS concentration was significantly reduced(P<0.05),and the concentration of serum proinflammatory factors IL-6,TNF-α,IFN-γand IL-1βwere significantly reduced(P<0.05),while the concentration of antiinflammatory factors IL-4,IL-5,IL-10,and IL-13 increased significantly(P<0.05),the concentration of chemokine KC/GRO decreased significantly(P<0.05).Conclusion:Astragalus could promote the activity of intestinal alkaline phosphatase and effectively regulate blood sugar and inflammation in T2 DM mice.

关 键 词：炎症 糖尿病 黄芪 炎症因子 

分 类 号：TS201.4[轻工技术与工程—食品科学]