微拟球藻总脂中甘油酯组成定量分析及EPA的存在形式研究  

Quantitative analysis of glycerides composition and existence form of EPA in total lipid of Nannochloropsis sp.

作　　者：黎崎均 周真真 张东梅[1] 丛威[1] LI Qijun;ZHOU Zhenzhen;ZHANG Dongmei;CONG Wei(State Key Laboratory of Biochemical Engineering,Chinese Academy of Sciences Institute of Process Engineering,Beijing 100190,China;School of Chemical Engineering,University of Chinese Academy of Sciences,Beijing 100190,China)

机构地区：[1]中国科学院过程工程研究所生化重点实验室,北京100190 [2]中国科学院大学化学工程学院,北京100190

出　　处：《中国油脂》2021年第6期119-124,共6页China Oils and Fats

基　　金：“十三五”国家重点研发计划(2018YFD0401105)。

摘　　要：采用正己烷-乙醇混合溶剂提取微拟球藻总脂,分级后得到中性脂、糖脂和磷脂,采用薄层层析分离3组分,并采用气相色谱法定量分析各组分在总脂中的含量及脂肪酸组成,同时分析了EPA在各脂质中的分布。结果表明:微拟球藻总脂甘油酯的中性脂、糖脂、磷脂占比分别为33.42%、38.22%和28.36%;中性脂主要组分为甘油三酯(TAG)和游离脂肪酸(FFA),在总脂中的含量分别为9.27 g/100 g和5.79 g/100 g;糖脂主要组分为单半乳糖甘油二酯(MGDG)和双半乳糖甘油二酯(DGDG),在总脂中的含量分别为14.03 g/100 g和5.81 g/100 g;磷脂主要组分为磷脂酰乙醇胺(PE)、磷脂酰胆碱(PC)和磷脂酰肌醇(PI),在总脂中的含量分别为6.22、3.53 g/100 g和1.53 g/100 g。3类脂质之和占微拟球藻总脂的51.49%;不同脂类脂肪酸组成差别较大,EPA在甘油一酯(MAG)、MGDG、DGDG和PE中比例较高,分别占各组分脂肪酸总量的16.94%、37.24%、14.32%和10.51%,EPA在中性脂中分布最低,83.59%的EPA以糖脂形式存在。The total lipid was extracted from Nannochlopsis sp.by mixed n-hexane and ethanol solvent,neutral lipid,glycolipid and phospholipid were obtained after lipid fractionation,the components in the three lipids were separated by thin-layer chromatography,and quantitatively determined with gas chromatography,and the fatty acid compositions of the components in neutral lipid,glycolipid and phospholipid were also determined.At the same time,the distribution of EPA in each lipid was analyzed.The results showed that the proportions of neutral lipids,glycolipids and phospholipids in glycerides of total lipid of Nannochlopsis sp.were 33.42%,38.22%and 28.36%,respectively.The main components of neutral lipids were triacylglycerol(TAG)and free fatty acid with the contents of 9.27 g/100 g and 5.79 g/100 g respectively in the total lipid;the main components of glycolipids were medium monogalactose diglyceride(MGDG)and digalactosyl diglyceride(DGDG)with the contents of 14.03 g/100 g and 5.81 g/100 g,respectively in the total lipid;the main components of phospholipids were phosphatidylethanolamine(PE),phosphatidycholine(PC)and phosphatidylinositol(PI)with the contents of 6.22,3.53 g/100 g and 1.53 g/100 g,respectively in the total lipid.The absolute total content of the three lipids in total lipid was 51.49 g/100 g.The fatty acid composition of different lipids was quite different.EPA had a higher proportion in monoglyceride(MAG),MGDG,DGDG and PE,accounting for 16.94%,37.24%,14.32%and 10.51%of the total fatty acids of each component,respectively.EPA had the lowest distribution in neutral lipids,and 83.59%of EPA existed in the form of glycolipids.

关 键 词：微拟球藻 油脂分析 薄层层析 EPA分布 

分 类 号：Q936[生物学—微生物学] TQ646[化学工程—精细化工]