酶解猪血制备氨基酸液体肥工艺条件优化  

Optimization of technological conditions for preparing amino acid liquid fertilizer by enzymatic hydrolysis of pig blood

作　　者：蔡艺菲 赵鑫阳 田晓静[1,2] 宋礼 罗丽 丁功涛 曹竑 陈士恩[1,2] Cai Yifei;Zhao Xinyang;Tian Xiaojing;Song Li;Luo Li;Ding Gongtao;Cao Hong;Chen Shien(College of Life Science and Engineering,Northwest Minzu University,Gansu Lanzhou 730124;China-Malaysia National Joint Lab,Biomedical Research Center,Northwest Minzu University,Gansu Lanzhou 730030;Gannan Research Institute of Yak Milk,Gansu Gannan 747000)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730124 [2]西北民族大学生物医学研究中心中国-马来西亚国家联合实验室,甘肃兰州730030 [3]甘南牦牛乳研究院,甘肃甘南747000

出　　处：《现代畜牧兽医》2021年第6期23-26,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：科技助力经济2020重点专项(SQ2020YFF0405583);西北民族大学中央高校基本科研业务费资助项目(31920200115、31920190022)。

摘　　要：试验以猪血为原料,利用风味蛋白酶水解猪血中的蛋白,以水解率为指标,得到酶解血液的最佳条件。通过单因素试验研究料液比、酶添加量、pH值、温度和水解时间对猪血蛋白水解率的影响;正交试验确定水解的较佳工艺条件为:料液比1∶10.5 g/mL、pH值6、水解温度50℃、酶浓度12000 U/g蛋白、水解时间7 h。在此条件下,水解率为40.42%。In the study,porcine blood was used as the raw material,and flavored protease was used to hydrolyze porcine hemoglobin.The degree of hydrolysis was used as an index to obtain the best conditions for enzymatic hydrolysis of blood.The influence of material-liquid ratio,enzyme addition amount,pH value,temperature,and hydrolysis time on the hemoglobin hydrolysis rate was studied through a single factor experiment.Orthogonal experiments were performed to determine the optimal process conditions for the hydrolysis:the material-liquid ratio 1∶10.5 g/mL,pH value 6,hydrolysis temperature 50℃,enzyme concentration 12000 U/g protein,hydrolysis time 7 h,under these conditions the hydrolysis rate was 40.42%.

关 键 词：猪血红蛋白 风味蛋白酶 水解率 

分 类 号：S879.9[农业科学—畜牧兽医]