干酪乳杆菌Zhang在逆境条件下基因表达的差异分析  

Analysis of Differential Gene Expression of Lactobacillus casei Zhang under Adverse Circumstances

作　　者：张猛 贾星 张和平[1] 马学波 包秋华[1] Zhang Meng;Jia Xing;Zhang Heping;Ma Xuebo;Bao Qiuhua(Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Inner Mongolia Agricultural University,Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Hohhot 010018)

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室农业农村部奶制品加工重点实验室,呼和浩特010018

出　　处：《中国食品学报》2021年第6期62-69,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金地区科学基金项目(31860434);内蒙古自然科学基金项目(2019MS03051);国家自然科学基金青年科学基金项目(31301516)。

摘　　要：为了探讨益生菌干酪乳杆菌Zhang在低温、低pH值胁迫下转录水平上的基因表达差异。本文选取干酪乳杆菌Zhang中与糖代谢、氨基酸代谢、细胞膜脂肪酸以及应激蛋白等相关的12个主要基因为研究对象,将干酪乳杆菌Zhang用MRS液体培养基活化3代后置于4℃条件下进行逆境胁迫,间断性取样,采用反转录PCR对选定基因的表达水平进行动态监测。结果表明,干酪乳杆菌Zhang在低温、低pH值胁迫下,3个糖代谢相关基因(苹果酸乳酸酶基因、6-磷酸葡萄糖脱氢酶基因、UTP-葡萄糖-1-磷酸尿苷酰转移酶)、1个细胞膜脂肪酸相关基因(环丙烷脂肪酸合成酶基因)、1个应激蛋白相关基因(分子伴侣Gro EL基因)共5个基因表达上调;而DNA引发复制酶基因、3个氨基酸代谢相关基因(阳离子转运酶基因、D-谷氨酸代谢基因、ABC转运体ATP结合蛋白基因)、2个细胞膜脂肪酸基因(磷脂合成基因、短链醇脱氢酶基因)、磷酸转移酶系统丝氨酸特异性转运蛋白基因等7个基因表达下降。这为解析干酪乳杆菌Zhang在胁迫条件下的生理、生化特性的改变及其抗性机制提供了参考依据。This work aimed to study differential gene expression at transcriptional level of probiotics Lactobacillus casei Zhang growth under low temperature and low pH conditions.In this study,Lactobacillus casei Zhang was cultured in MRS liquid medium for three generations and grown under cold stress and low pH environments at 4℃.Samples were taken intermittently.Twelve Lactobacillus casei Zhang genes,including genes associated with carbohydrate metabolism,amino acid metabolism,cell membrane fatty acid,and stress protein were continuously monitored under the adverse growth condition by reverse transcription polymerase chain reaction(PCR).The results showed that five genes were up-regulated under the adverse circumstances,including three genes related to glucose metabolism(NAD-dependent malic enzyme,glucose-6-phosphate1-dehydrogenase,UTP-glucose-1-phosphate uridylyltransferase),one gene encoding a cell membrane fatty acid synthesis enzyme(cyclopropane fatty acid synthase),and one gene encoding a stress protein(chaperonin Gro EL).While the expression of seven genes was down-regulated,including the gene coding a DNA primase,three amino acid metabolism-related genes(cation transport ATPase,D-glutamic acid metabolism,ABC transporter ATP-binding protein),two membrane fatty acid genes(phospholipid biosynthesis protein,short-chain alcohol dehydrogenase),and PTS cellobiose-specific transporter subunit IIA.This work provides some references for analyzing the changes in physiological and biochemical characteristics,as well as stress resistance mechanism of Lactobacillus casei Zhang when grown under adverse growth condition.

关 键 词：干酪乳杆菌Zhang 逆境胁迫 差异基因 反转录qPCR 

分 类 号：TS201.3[轻工技术与工程—食品科学]