Adenovirus-mediated LIGHT gene modification in murine B-cell lymphoma elicits a potent antitumor effect  

作　　者：Guili Hu Yang Liu Hong Li Dekuang Zhao Liuqing Yang Jiangen Shen Xuejun Hong Xuetao Cao Qingqing Wang 

机构地区：[1]Institute of Immunology,Zhejiang University,Hangzhou,China [2]Molecular and Cellular Biology Group,Laboratory of Respiratory Biology,NIEHS/NIH,Research Triangle Park,NC,USA [3]National Key Laboratory of Medical Immunology&Institute of Immunology,Second Military Medical University,Shanghai,China

出　　处：《Cellular & Molecular Immunology》2010年第4期296-305,共10页中国免疫学杂志（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(30328011 and 30872377);the National Basic Research Program of China(2004CB518802);the Science and Technology Department of Zhejiang Province(2008C23044).

摘　　要：Here,we investigated the antitumor effect of adenovirus-mediated gene transfer of LIGHT,the tumor-necrosis factor(TNF)superfamily member also known as TNFSF14,in the murine A20 B-cell lymphoma.LIGHT gene modification resulted in upregulated expression of Fas and the accessory molecule-intercellular adhesion molecule-1(ICAM-1)on A20 cells and led to enhanced A20 cell apoptosis.LIGHT-modified A20 cells effectively stimulated the proliferation of T lymphocytes and interferon(IFN)-c production in vitro.Immunization of BALB/c mice with a LIGHT-modified A20 cell vaccine efficiently elicited protective immunity against challenge with the parental tumor cell line.Adenovirus-mediated gene transfer of LIGHT by intratumoral injection exerted a very potent antitumor effect against pre-existing A20 cell lymphoma in BALB/c mice.This adenovirus-mediated LIGHT therapy induced substantial splenic natural killer(NK)and cytotoxic T lymphocyte(CTL)activity,enhanced tumor infiltration by inflammatory cells and increased chemokine expression of CC chemokine ligand 21(CCL21),IFN-inducible protein-10(IP-10)and monokine induced by IFN-c(Mig)from tumor tissues.Thus,adenovirus-mediated LIGHT therapy might have potential utility for the prevention and treatment of B-cell lymphoma.

关 键 词：ADENOVIRUS B lymphoma gene transfer LIGHT/TNFSF14 

分 类 号：R73[医药卫生—肿瘤]