Evaluation of Various Factors Affecting Bioconversion of L-Tyrosine to L-DOPA by Yeast Yarrowia lipolytica-NCIM 3450 Using Response Surface Methodology  

作　　者：Swati T.Gurme Shripad N.Surwase Sushama A.Patil Jyoti P.Jadhav 

机构地区：[1]Department of Biotechnology,Shivaji University,Vidyanagar,Kolhapur 416004,India [2]Department of Microbiology,Shivaji University,Kolhapur 416004,India

出　　处：《Natural Products and Bioprospecting》2014年第3期141-147,共7页应用天然产物（英文）

摘　　要：3,4-Dihydroxy L-phenylalanine(L-DOPA)is considered a potent drug for the treatment of Parkinson disease.Physical and nutritional parameters where optimized by using Yarrowia lipolytica-NCIM 3450 to accomplished the highest production of L-DOPA.Screenings of critical components were completed by using a Plackett–Burman design,while further optimization was carried out using the Box–Behnken design.The optimized factor levels predicted by the model were pH 6.1,1.659 g L^(-1)yeast extract,1.491 g L^(-1)L-tyrosine and 0.0290 g L^(-1)CuSO4.The predicted yield of L-DOPA with these levels was 1.319 g L^(-1),while actual yield obtained was 1.273 g L^(-1).The statistical analysis revealed that model is significant with F value 19.55 and R2 value 0.9514.This process resulted in a 3.594-fold increase in the yield of LDOPA.L-DOPA was confirmed by HPTLC and HPLC analysis.Thus,Yarrowia lipolytica-NCIM 3450 has potential to be a new source for the production of L-DOPA.

关 键 词：L-DOPA L-TYROSINE RSM Yarrowia lipolytica 

分 类 号：R74[医药卫生—神经病学与精神病学]