A dimeric structure of PD-L1: functional units or evolutionary relics?  

作　　者：Yong Chen Peipei Liu Feng Gao Hao Cheng Jianxun Qi George F.Gao 

机构地区：[1]CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences(CASPMI),Beijing 100101,China [2]College of Life Sciences,Graduate University,Chinese Academy of Sciences,Beijing 100049,China [3]Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [4]China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Protein & Cell》2010年第2期153-160,共8页蛋白质与细胞（英文版）

基　　金：This work was supported by a grant from Ministry of Science and Technology(MOST)of China for the basic research program 973,Grant No.2006CB504204;a grant from National Natural Science Foundation(NSFC)of China,Grant No.30671903;a grant from Chinese Academy of Sciences(CAS)Knowledge Innovation Project,Grant No.KSCX2-SW-227;G.F.G.is a distinguished young investigator of the NSFC(Grant No.30525010);Y.C.is a Ph.D.student supported by Science Innovation Project,Graduate University of Chinese Academy of Sciences(GUCAS),No.0729031EE1;The China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology is partlially supported by Japan MEXT(Ministry of Education,Culture,Sports,Science and Technology).

摘　　要：PD-L1 is a member of the B7 protein family,most of whose members so far were identified as dimers in a solution and crystalline state,either complexed or uncomplexed with their ligand(s).The binding of PD-L1 with its receptor PD-1(CD279)delivers an inhibitory signal regulating the T cell function.Simultaneously with the Garboczi group,we successfully solved another structure of human PD-L1(hPD-L1).Our protein crystallized in the space group of C222_(1) with two hPD-L1 molecules per asymmetric unit.After comparison of reported B7 structures,we have found some intrinsic factors involved in the interaction of these two molecules.Based on these results,we tend to believe this uncomplexed hPD-L1 structure demonstrated its potential dimeric state in solution,althougt it could just be an evolutionary relic,too weak to be detected under present technology,or still a functional unit deserved our attentions.

关 键 词：PD-L1 crystal structure DIMER costimulatory molecules B7 family 

分 类 号：R73[医药卫生—肿瘤]