HID-1 is a peripheral membrane protein primarily associated with the medial-and transGolgi apparatus  

作　　者：Lifen Wang Yi Zhan Eli Song Yong Yu Yaming Jiu Wen Du Jingze Lu Pingsheng Liu Pingyong Xu Tao Xu 

机构地区：[1]National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [2]Graduate School of the Chinese Academy of Sciences,Beijing 100864,China [3]College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China

出　　处：《Protein & Cell》2011年第1期74-85,共12页蛋白质与细胞（英文版）

基　　金：the National Science Foundation of China(Grant Nos.30870564,and 30900268),The Beijing Natural Science Foundation(No.5092017);the Major State Basic Research Program of China(No.2010CB833701);the CAS Project(KSCX2-SW-224 and Novo Nordisk-CAS to P Xu).

摘　　要：Caenorhabditis elegans hid-1 gene was first identified in a screen for mutants with a high-temperature-induced dauer formation(Hid)phenotype.Despite the fact that the hid-1 gene encodes a novel protein(HID-1)which is highly conserved from Caenorhabditis elegans to mammals,the domain structure,subcellular localization,and exact function of HID-1 remain unknown.Previous studies and various bioinformatic softwares predicted that HID-1 contained many transmembrane domains but no known functional domain.In this study,we revealed that mammalian HID-1 localized to the medial-and transGolgi apparatus as well as the cytosol,and the localization was sensitive to brefeldin A treatment.Next,we demonstrated that HID-1 was a peripheral membrane protein and dynamically shuttled between the Golgi apparatus and the cytosol.Finally,we verified that a conserved N-terminal myristoylation site was required for HID-1 binding to the Golgi apparatus.We propose that HID-1 is probably involved in the intracellular trafficking within the Golgi region.

关 键 词：HID-1 GOLGI peripheral membrane protein fluorescent recovery after photobleaching N-MYRISTOYLATION 

分 类 号：R73[医药卫生—肿瘤]