Inhibition of SIRT6 in prostate cancer reduces cell viability and increases sensitivity to chemotherapeutics  

作　　者：Yewei Liu Qian Reuben Xie Boshi Wang Jiaxiang Shao Tingting Zhang Tengyuan Liu Gang Huang Weiliang Xia 

机构地区：[1]School of Biomedical Engineering and Med-X Research Institute,Shanghai Jiao Tong University,Shanghai 200030,China [2]De partment of Nuclear Medicine,Renji Hospital,Sh anghai Jiao Tong University School of Medicine,Shanghai 200127,China [3]C linical Stem Cell Center,Renji Hospital,Sh anghai Jiao Tong University School of Medicine,Shanghai 200127,China

出　　处：《Protein & Cell》2013年第9期702-710,共9页蛋白质与细胞（英文版）

基　　金：The study was supported by research grants from National Natural Science Foundation of China(Grant Nos.30830038,30970842,81071180,30900756 and 31270032);the National Basic Research Program(973 Program)(No.2012CB932604);New Drug Discovery Project(2012ZX09506-001-005);Key Project of Science and Tech-nology Commission of Shanghai Municipality(No.10JC1410000);Shanghai Leading Academic Discipline Project(No.S30203)。

摘　　要：SI RT6 is an important histone modifying protein that regulates DNA repair,telomere maintenance,energy me-tabolism,and target gene expression.Recently SIRT6 has been identifi ed as a tumor suppressor and is down-regulated in certain cancer types,but not in other can-cers.From deposited gene profi ling studies we found that SIRT6 was overexpressed in prostate tumors,compared with normal or paratumor prostate tissues.Tissue micro-array studies confi rmed the higher levels of SIRT6 in both prostate tumor tissues and prostate cancer cells than in their normal counterparts.Knockdown of SIRT6 in human prostate cancer cells led to sub-G1 phase arrest of cell cy-cle,increased apoptosis,elevated DNA damage level and decrease in BCL2 gene expression.Moreover,SIRT6-de-fi ciency reduced cell viability and enhanced chemothera-peutics sensitivity.Taken together,this study provides the fi rst evidence of SIRT6 overexpression in human prostate cancer,and SIRT6 regulation could be exploited for pros-tate cancer therapy.

关 键 词：SI RT6 OVEREXPRESSION prostate cancer th erapy 

分 类 号：R73[医药卫生—肿瘤]