CD1d^(hi)CD5^(+)B cells differentiate into antibody-secreting cells under the stimulation with calreticulin fragment  

作　　者：Tengteng Zhang Yun Xia Lijuan Zhang Wanrong Bao Chao Hong Xiao-Ming Gao 

机构地区：[1]Soochow University,Institutes of Biology and Medical Sciences,Suzhou 215123,China

出　　处：《Protein & Cell》2013年第11期872-881,共10页蛋白质与细胞（英文版）

基　　金：This study was supported by grants from PCSIRT(IRT1075);the National Natural Science Foundation of China(Grant Nos.31370908,31070781,and 31100633);the National Basic Research Program(973 Program)(No.2010CB529102);Foundation of Nature Science of Jiangsu Higher Education Institutions of China(11KJB180011).

摘　　要：Calreticulin(CRT)is a multifunctional molecule in both intracellular and extracellular environment.We have previ-ously found that a recombinant CRT fragment(rCRT/39-272)could modulate T cell-mediated immunity in mice via activation and expansion of CD1dhiCD5+B cells as well as induction of CRT-specifi c regulatory antibodies.Anti-body secreting cells(ASCs)are terminally differentiated B cells responsible for producing antibodies to participate in positive immune response as well as immune regula-tion.In this study,we demonstrate that rCRT/39-272 dif-ferentiates murine CD1dhiCD5+B cells into ASCs mark-ed by increased expression of plasma cell-associated transcription factors and production of polyreactive antibodies against DNA and CRT in vitro.Intraperitoneal administration of rCRT/39-272 augmented differentiation of CD1dhiCD5+B cells into ASCs in naïve mice or mice with experimental autoimmune encephalomyelitis.Thus,we propose that ASC differentiation and subsequent an-tibody production of CD1dhiCD5+B cells are key steps in CRT-mediated immunoregulation on infl ammatory T cell responses.

关 键 词：CALRETICULIN regulatory B cells antibody se-creting cells 

分 类 号：R73[医药卫生—肿瘤]