羧甲基茯苓多糖的水媒法制备及其免疫活性研究  

Carboxymethyl-pachymaran from the fruiting body of Poria cocos: preparation in aqueous system and its immunomodulation activity

作　　者：刘星汶 杨继国[1] 徐晓飞 LIU Xing-Wen;YANG Ji-Guo;XU Xiao-Fei(College of Food Science and Engineering,South China University of Technology,Guangzhou,Guangdong 510640,China;South China Institute of Collaborative Innovation,Dongguan,Guangdong 523808,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510640 [2]华南协同创新研究院,广东东莞523808

出　　处：《菌物学报》2021年第6期1575-1582,共8页Mycosystema

基　　金：国家重点研发计划(2018YFC1602102);中国博士后科学基金(2016T90787)。

摘　　要：羧甲基茯苓多糖carboxymethyl-pachymaran(CMP)具有良好的免疫调节活性,传统方法使用两步工序在醇相体系中制得,有机溶剂消耗大、成本高。本研究报道一种新制备方法,将提取茯苓多糖以及茯苓多糖与氯乙酸的醚化反应整合在一个碱液体系中完成。动物实验表明,新方法制备的CMP能提高环磷酰胺诱导的免疫低下小鼠的细胞免疫和体液免疫功能,对S180肉瘤具有抑制活性。经口急性毒理结果显示CMP属于实际无毒物质,适合于功能性食品和保健食品开发应用。Traditionally, the immunomodulatory carboxymethyl-pachymaran(CMP) polysaccharide was prepared in a two-step process in alcohol solution. However, this process consumes a large amount of organic solvents and is not cost-effective. In the present study, a new method for preparing CMP is reported. The extractions of polysaccharides from the fruiting body of Poria cocos and the etherification of polysaccharides with chloroacetic acid were integrated into one-step process in aqueous alkaline solution. All the same, the CMP prepared by the new method improved cellular and humoral immune responses in cyclophosphamide-induced immunosuppressed mice and exhibited anti-tumor activity in S180-bearing mice. Oral acute toxicological results demonstrate that CMP is a non-toxic substance, and suitable for the development of functional foods in food industry.

关 键 词：羧甲基茯苓多糖 抗肿瘤 免疫调节 水相体系 

分 类 号：TS201.2[轻工技术与工程—食品科学] R285.5[轻工技术与工程—食品科学与工程]