Fast saccharide mapping method for quality consistency evaluation of commercial xylooligosaccharides collected in China  

作　　者：Yong Deng Cunwu Chen Lingxiao Chen Bangxing Han Shaoping Li Jing Zhao 

机构地区：[1]State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macao,Macao,China [2]Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources,West Anhui University,Lu'an,Anhui,China

出　　处：《Journal of Pharmaceutical Analysis》2021年第3期284-291,共8页药物分析学报（英文版）

基　　金：funded by grants from the National Natural Science Foundation of China(No.81673389);the National Key R&D Program of China(2019YFC1711300);the Science and Technology Development Fund,Macao SAR(File no.0075/2018/A2,034/2017/A1 and 0017/2019/AKP);the University of Macao(File no.MYRG2018-00083-ICMS,MYRG2019-00128-ICMS,CPG2020-00021-ICMS)。

摘　　要：Due to the extensive use of xylooligosaccharides(XOS)as functional food ingredients,many inferior goods and even adulterants are generally found in the market,which may pose a health hazard to certain populations.Chromatography method such as high-performance liquid chromatography(HPLC)and high-performance thin-layer chromatography(HPTLC)is traditionally applied for the quality analysis of XOS.However,it is time consuming due to the prolonged separation and pre-or post-derivatization procedure.In this study,a fast saccharide mapping method based on matrix-assisted laser desorption/time-of-flight mass spectrometry(MALDI-TOF-MS)was developed for the quality consistency analysis of 22 batches of XOS collected from different manufacturers in China.The time needed for saccharides analysis using MALDI-MS was less than 30 min for one plate,at least 6 times faster than that by the traditional HPTLC chromatography method.In addition,MALDI-MS possessed higher resolution for XOS with DP4-DP7 based on the difference of m/z,which is hardly separated using HPTLC.The results showed that XOS were present only in samples XY01-XY11,samples XY12-XY14 only consisted of hex oligosaccharides,and samples XY15-XY22 were free of oligosaccharides.These indicate that the quality consistency of XOS products in the China market was poor,which should be carefully investigated.

关 键 词：XYLOOLIGOSACCHARIDES Saccharide mapping MALDI-MS Quality control 

分 类 号：TS201.2[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]