A novel aptamer-based histochemistry assay for specific diagnosis of clinical breast cancer tissues  

作　　者：Mei Liu Lei Xi Ting Tan Lian Jin Zhifei Wang Nongyue He 

机构地区：[1]State Key Laboratory of Bioelectronics,National Demonstration Center for Experimental Biomedical Engineering Education(Southeast University),School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China [2]School of Chemistry and Chemical Engineering,Southeast University,Nanjing 211189,China [3]Department of Pathology,The First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China [4]Department of Pathology.Hunan Provincial Maternal and Child Health Care Hospital,Changsha 410008,China [5]Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province,Hunan Key Laboratory of Biomedical Nanomaterials and Devices,Hunan University of Technology,Zhuzhou 412007,China

出　　处：《Chinese Chemical Letters》2021年第5期1726-1730,共5页中国化学快报（英文版）

基　　金：supported by the National Key Research and Development Program of China (No.2017YFA0205301);the National Natural Science Foundation of China (Nos.61527806, 81902153 and 81771976);the Scientific Research Foundation of the Graduate School of Southeast University (No.YBPY1881)

摘　　要：Pathological detection using immunohistochemistry(IHC)has become an indispensable process in the diagnosis confirmation of various cancers.However,the production of monoclonal antibodies is always very complex,expensive and time-consuming,and the batch differences are significant due to the corporeity and health statuses of animals may be different.In this work,an aptamer-based histochemistry(aptahistochemistry)assay was developed using a DNA aptamer for specific diagnosis of clinical breast cancer tissue sections.This aptahistochemistry assay can specifically distinguish Luminal A breast cancer molecular subtype from Luminal B(HER2+),HER2-enriched,and triple-negative breast cancer molecular subtypes,as well as para-carci noma tissue,mastitis tissue and normal breast tissue.The accuracy of this aptahistochemistry assay for the diagnosis of Luminal A breast cancer was as high as 80%,which showed a great potential for clinical pathological diagnosis applications.

关 键 词：APTAMER IMMUNOHISTOCHEMISTRY Breast cancer Molecular subtype 

分 类 号：R737.9[医药卫生—肿瘤]