耐盐碱水稻土壤产甲烷菌群落特征及产甲烷途径  

Community Characteristics of Methanogens and Methanogenic Pathways in Salttolerant Rice Soil

作　　者：杨雨虹 贺惠[4] 米铁柱[1,2,3] 刘玥腾 刘佳音 张国栋 李明月 甄毓 YANG Yu-hong;HE Hui;MI Tie-zhu;LIU Yue-teng;LIU Jia-yin;ZHANG Guo-dong;LI Ming-yue;ZHEN Yu(Key Laboratory of Marine Environment and Ecology,Ministry of Education,Ocean University of China,Qingdao 266100,China;Laboratory for Marine Ecology and Environmental Science,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China;College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China;College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China;Qingdao Salt and Alkali Rice Research and Development Center,Qingdao 266041,China;Jiutian Wisdom Agricultural Group,Qingdao 266003,China;School of Resources and Environmental Engineering,Shandong University of Technology,Zibo 255049,China)

机构地区：[1]中国海洋大学海洋环境与生态教育部重点实验室,青岛266100 [2]青岛海洋科学与技术国家实验室,海洋生态与环境科学功能实验室,青岛266237 [3]中国海洋大学环境科学与工程学院,青岛266100 [4]中国海洋大学海洋生命学院,青岛266003 [5]青岛海水稻研究发展中心,青岛266041 [6]九天智慧农业集团,青岛266003 [7]山东理工大学资源与环境工程学院,淄博255049

出　　处：《环境科学》2021年第7期3472-3481,共10页Environmental Science

基　　金：山东省博士后创新项目(201702016);青海省科技计划项目(2020-NK-168);青岛创业创新领军人才计划项目(18-1-2-13-2hc)。

摘　　要：产甲烷菌在土壤碳循环过程中发挥着关键性作用,而种植耐盐碱水稻土壤中产甲烷菌群落特征及环境影响因素尚不明确.本研究采用实时荧光定量PCR和Illumina高通量测序技术,对青岛市稻作改良智慧农业产业示范基地中耐盐碱水稻(YC1703)、普通水稻("临稻10号")及荒地土壤中产甲烷菌的丰度特征、群落组成及与环境因子的关系进行了对比分析.结果表明,种植"临稻10号"土壤中的产甲烷菌丰度和群落丰富度显著高于种植YC1703的土壤,种植YC1703土壤中产甲烷菌具有较高多样性.产甲烷菌群落受水稻品种、水稻生育期和环境因子的综合影响.两种水稻土壤中H2/CO_(2)还原型产甲烷菌占优势,由此推测这些土壤中生成甲烷的主导途径均为H2/CO_(2)还原途径.It is known that methanogens play a critical role in the carbon cycle in soil,while methanogen community characteristics and their environmental influencing factors in the soil planted with salt-tolerant rice remain unclear.In this study,methanogen abundance,community composition,and relationships with environmental factors in soils planted with the salt-tolerant rice (YC1703) and ordinary rice (Lindao 10) were evaluated in the rice improvement demonstration base of Qingdao Wisdom Agricultural Industry using real-time fluorescence quantitative PCR and Illumina high-throughput sequencing.The results indicated that the abundance and community richness of methanogens in Lindao 10 soil were significantly higher than those in YC1703 soil,and methanogens in YC1703 soil exhibited higher diversity.The combined effects of rice varieties,rice growth period,and environmental factors had impacts on the methanogen community.The hydrogenotrophic methanogens were dominant in the YC1703 and Lindao 10 soils;thus,we speculated that the dominant pathway of methane production in these soils was hydrogenotrophic methanogenesis.

关 键 词：产甲烷菌 水稻 碳循环 土壤 H2/CO2还原型 

分 类 号：X172[环境科学与工程—环境科学]