玉米品种和淀粉老化处理对其红外光谱结构与体外发酵参数的影响  

Effects of Corn Variety and Starch Retrogradation on Infrared Spectroscopy Structure and in Vitro Fermentation Parameters

作　　者：陈凯 江为民 贺志雄[1,4] 贺建华 谭支良[1,4] CHEN Kai;JIANG Weimin;HE Zhixiong;HE Jianhua;TAN Zhiliang(Central South Scientific Observing and Experimental Station of Animal Nutrition and Feed,Ministry of Agriculture,Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Key Laboratory for Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha 410125,China;College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China;Hunan Institute of Animal and Veterinary Science,Changsha 410131,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,畜禽养殖污染控制与资源化技术国家工程实验室,湖南省动物营养生理与代谢过程重点实验室,农业部中南动物营养与饲料科学观测实验站,长沙410125 [2]湖南农业大学动物科学技术学院,长沙410128 [3]湖南省畜牧兽医研究所,长沙410131 [4]中国科学院大学,北京100049

出　　处：《动物营养学报》2021年第7期3980-3993,共14页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：湖南创新型省份建设专项经费资助(湖南省重点研发计划项目2020NK2049,创新省份建设专项2019RS3021);中科院STS(KFJ⁃STS⁃ZDTP⁃075)。

摘　　要：本试验旨在研究不同玉米品种[普通玉米和高直链淀粉玉米(简称高直玉米)]和淀粉老化处理对其营养成分、傅里叶变换红外光谱(FTIR)分子结构以及体外发酵参数的影响,并探索玉米FTIR分子结构参数与体外发酵特征的相关关系。结果表明,玉米品种和淀粉老化处理对部分FTIR结构参数、体外发酵总挥发性脂肪酸(VFA)含量和丁酸占比存在显著交互作用(P<0.05),老化前高直玉米β-葡聚糖峰高、纤维化合物面积和峰高以及碳水化合物Ⅲ区面积显著高于普通玉米(P<0.05);老化后高直玉米的丁酸占比显著低于普通玉米(P<0.05),老化后高直玉米总VFA含量和丁酸占比显著低于老化前普通玉米(P<0.05),但是老化后高直玉米与普通玉米在FTIR分子结构方面没有显著差异(P>0.05)。高直玉米丙酸占比显著高于普通玉米(P<0.05),而总产气量、甲烷产气量和氢气产气量显著低于普通玉米(P<0.05);淀粉老化处理显著降低了总产气量、甲烷产气量、氢气产气量、干物质降解率和丙酸占比(P<0.05),而显著提高了乙酸占比(P<0.05)。相关性分析结果显示,总产气量与酰胺Ⅰ区峰高、酰胺Ⅰ区与酰胺Ⅱ区峰高比、纤维化合物峰高以及总碳水化合物区域结构呈显著负相关(P<0.05),与酰胺Ⅱ区面积和峰高呈显著正相关(P<0.05);干物质降解率与酰胺Ⅱ区峰高、酰胺Ⅰ区与酰胺Ⅱ区峰高比、纤维化合物峰高、碳水化合物Ⅰ区面积、碳水化合物Ⅰ区与Ⅱ区峰高呈显著负相关(P<0.05);乙酸占比与酰胺Ⅰ区峰高、酰胺Ⅰ区与酰胺Ⅱ区峰高比、纤维化合物峰高和总碳水化合物区域结构呈显著正相关(P<0.05),与β-葡聚糖面积和峰高呈显著负相关(P<0.05);总VFA含量与酰胺Ⅱ区面积和峰高呈显著正相关(P<0.05),与酰胺Ⅰ区峰高、酰胺Ⅰ区与酰胺Ⅱ区面积比和峰高比、纤维化合物峰高以及总碳水化合物区域结构呈显著负相关(P<0.05)。�The experiment was conducted to analyze different corn varieties(normal corn and high amylose corn)and starch retrogradation on feed nutrients,Fourier transform infrared spectroscopy(FTIR)molecular structure and in vitro fermentation parameters,and to explore the correlation between FTIR molecular structure parameters and in vitro fermentation characteristics of corns.The results showed that there were significant in⁃teractions between corn variety and starch retrogradation on sectional FTIR structure parameters,total volatile fatty acid(VFA)content and the butyrate proportion in in vitro fermentation(P<0.05).Compared with the normal corn,theβ⁃glucan peak height,the area and peak height of cellulosic compounds and carbohydrate peakⅢarea in the high amylose corn before retrogradation were significantly increased(P<0.05),but the bu⁃tyrate proportion in the high amylose corn after retrogradation was significantly decreased(P<0.05).Com⁃pared with the normal corn before retrogradation,total VFA content and the butyrate proportion in the high am⁃ylose corn after retrogradation were significantly reduced(P<0.05),but there was no significant difference in FTIR molecular structure between the normal corn and the high amylose corn after retrogradation(P<0.05).Compared with the normal corn,the propionate proportion in the high amylose corn was significantly increased(P<0.05),but the total gas production,methane production and hydrogen production were significantly re⁃duced(P<0.05).Starch retrogradation significantly reduced the total gas production,methane production,hy⁃drogen production,dry matter degradation rate and propionate proportion(P<0.05),and significantly in⁃creased the acetate proportion(P<0.05).The correlation analysis results showed that the total gas production was negatively correlated with the amideⅠpeak height,peak height ratio of amideⅠto amideⅡ,cellulosic compound peak height and total carbohydrate domain structure(P<0.05),while it was positively correlated with the area and peak

关 键 词：高直链淀粉玉米 傅里叶变换红外光谱结构 淀粉老化 体外发酵 相关性 

分 类 号：S816[农业科学—饲料科学]