响应面法优化酶解-超声辅助提取鸡血藤多糖工艺研究  

Optimization of enzymatic hydrolysis-ultrasonic assisted extraction of polysaccharides from spatholobi by response surface methodology

作　　者：丁梁斌[1] 马春梅[1] 赵苹苹 汤敏 DING Liangbin;MA Chunmei;ZHAO Pingping;TANG Min(School of Hygiene and Health,Xuzhou Vocational College of Bioengineering,Xuzhou 221006)

机构地区：[1]徐州生物工程职业技术学院卫生健康学院,徐州221006

出　　处：《中国食品添加剂》2021年第7期88-96,共9页China Food Additives

摘　　要：采用响应面法优选酶解-超声辅助提取鸡血藤多糖的工艺。在单因素试验的基础上,采用Box-Behnken响应面法考察酶添加量、酶解pH、超声时间和超声温度对鸡血藤多糖得率的影响。经多项式方程拟合,结果表明拟合方程显著,确定的鸡血藤多糖的最佳提取工艺为:酶添加量为0.15g、酶解pH为4.50、超声温度为52℃、超声时间36min。在此提取工艺下,鸡血藤多糖得率的预测试为9.42%。经验证试验,鸡血藤多糖实际得率为9.40%,与预测试接近,该工艺条件可为鸡血藤多糖的进一步研究和开发提供了依据。Response surface methodology was used to optimize the enzymatic hydrolysis-ultrasonic-assisted extraction process of polysaccharides from S.japonicum.On the basis of single factor experiment,Box-Behnken response surface method was used to investigate the effects of enzyme amount,enzymatic hydrolysis pH value,ultrasonic time and ultrasonic temperature on the yield of polysaccharide.The results of polynomial equation showed that the fitting equation was significant,and the optimum extraction process of polysaccharide was as follows:enzyme 0.15g,enzymolysis pH4.50,ultrasonic temperature 52℃,ultrasonic time 36 min.Under the above conditions,the predicted yield of polysaccharide was 9.42%.The experimental results showed that the actual yield of polysaccharide was 9.40% and was close to the prediction test.The process conditions can provide a basis for further research and development of polysaccharide.

关 键 词：鸡血藤 多糖 酶解 超声辅助 响应面法 

分 类 号：TS202.1[轻工技术与工程—食品科学]