高产蛋白酶菌株的分离、鉴定及酶学性质研究  

Isolation,identification and enzymatic properties of a high-yield protease strain

作　　者：冯菲[1] 权淑静[1] 刘丽[1] 王秋菊[1] 胡虹 解复红[1] FENG Fei;QUAN Shujing;LIU Li;WANG Qiuju;HU Hong;XIE Fuhong(Institute of Biology Co.,Ltd.,Henan Academy of Science,Zhengzhou 450008,China)

机构地区：[1]河南省科学院生物研究所有限责任公司,河南郑州450008

出　　处：《中国酿造》2021年第7期148-152,共5页China Brewing

基　　金：河南省科学院基本科研业务费项目(200605040);河南省科学院科技开放合作项目(210905010)。

摘　　要：该研究旨在从非传统环境(磷矿)中分离、筛选高产胞外蛋白酶菌株,对菌株进行形态学观察、生理生化和分子生物学鉴定,并研究其蛋白酶的酶学性质。结果表明,分离筛选到一株蛋白酶高产菌株,编号为PB5,该菌株被鉴定为贝莱斯芽孢杆菌(Bacillus velezensis),该菌株产胞外蛋白酶活力为562.3 U/mL,胞外蛋白酶的最适pH值为10,在pH 7~10有较好的pH稳定性;最适温度为60℃,20~50℃时有较好的稳定性;Na^(+)、Mg^(2+)、Mn^(2+)、K^(+)对蛋白酶活力有明显的促进作用;Fe^(2+)、Ag^(+)、乙二胺四乙酸(EDTA)、苯甲基磺酰氟(PMSF)、十二烷基硫酸钠(SDS)对蛋白酶活力有明显抑制效果。The purpose of this study was to isolate and screen high-yield extracellular protease strains from non-traditional environment(phosphate rock),the selected strain was identified by morphological observation and physiological characteristics as well as molecular biological identification.The results showed that a strain(numbered PB5)with high protease production was isolated and identified as Bacillus velezensis.The extracellular protease activity of the strain was 562.3 U/ml.The optimal pH value of extracellular protease was 10,and it had good pH stability between pH 7-10.The optimum temperature was 60℃,and it has good stability at 20-50℃.Na^(+),Mg^(2+),Mn^(2+),K^(+) had obvious promoting effect on protease activity.Fe^(2+),Ag^(+),ethylene diamine tetraacetic acid(EDTA),phenylmethanesulfonyl fluoride(PMSF)and sodium dodecyl sulfate(SDS)had obvious inhibitory effect on protease activity.

关 键 词：高产蛋白酶菌株 鉴定 酶学性质 

分 类 号：Q938.8[生物学—微生物学]