Lateral Hypothalamic Area Glutamatergic Neurons and Their Projections to the Lateral Habenula Modulate the Anesthetic Potency of Isoflurane in Mice  

作　　者：Shiyi Zhao Rui Li Huiming Li Sa Wang Xinxin Zhang Dan Wang Juan Guo Huihui Li Ao Li Tingting Tong Haixing Zhong Qianzi Yang Hailong Dong 

机构地区：[1]Department of Anesthesiology and Perioperative Medicine,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,China

出　　处：《Neuroscience Bulletin》2021年第7期934-946,共13页神经科学通报（英文版）

基　　金：the National Natural Science Foundation of China(81571351,81620108012,81671373,and 81771427);a Discipline Promotion Project of Xijing Hospital(XJZT18MJ33).

摘　　要：The lateral hypothalamic area(LHA)plays a pivotal role in regulating consciousness transition,in which orexinergic neurons,GABAergic neurons,and melanin-concentrating hormone neurons are involved.Glutamatergic neurons have a large population in the LHA,but their anesthesia-related effect has not been explored.Here,we found that genetic ablation of LHA glutamatergic neurons shortened the induction time and prolonged the recovery time of isoflurane anesthesia in mice.In contrast,chemogenetic activation of LHA glutamatergic neurons increased the time to anesthesia and decreased the time to recovery.Optogenetic activation of LHA glutamatergic neurons during the maintenance of anesthesia reduced the burst suppression pattern of the electroencephalogram(EEG)and shifted EEG features to an arousal pattern.Photostimulation of LHA glutamatergic projections to the lateral habenula(LHb)also facilitated the emergence from anesthesia and the transition of anesthesia depth to a lighter level.Collectively,LHA glutamatergic neurons and their projections to the LHb regulate anesthetic potency and EEG features.

关 键 词：ANESTHESIA ISOFLURANE Lateral hypothalamic area Lateral habenula Glutamatergic neuron 

分 类 号：R741[医药卫生—神经病学与精神病学]