Galangin and TRAIL cooperate to suppress A549 lung cancer proliferation via apoptosis and p38 MAPK activation  

作　　者：Wenjing Zhang Qilai Huang Zichun Hua 

机构地区：[1]The State Key Laboratory of Quality Research in Chinese Medicine,Faculty of Chinese Medicine,Macao University of Science and Technology and Macao Institute for Applied Research in Medicine,Macao,China [2]Changzhou High-Tech Research Institute of Nanjing University,Changzhou 213164,China [3]The State Key Laboratory of Pharmaceutical Biotechnology,Nanjing University,Nanjing 210093,China

出　　处：《Acta Pharmaceutica Sinica B》2012年第6期569-574,共6页药学学报（英文版）

基　　金：This study was supported,in part,by the Science and Technology Development Fund of the Macao Special Administrative Region(Nos.071/2009/A3 and 091/2009/A);the National Key Basic Research Project from the Chinese Ministry of Science and Technology(2012CB967004);the Chinese National Nature Sciences Foundation(81121062,50973046,31070706);the Jiangsu Provincial Nature Science Foundation(BK2010046,BZ2010074,BZ2011048,BK2011228,BZ2011048);the Bureau of Science and Technology of Changzhou(CN20100016,CZ20100008,CE20115034,CZ20110028).

摘　　要：Tumor necrosis factor-related apoptosis inducing ligand(TRAIL)is a promising antitumor therapy against lung cancer which is currently undergoing a phase II clinical trial in China.Unfortunately some cancer patients in the clinical trial displayed resistance to TR AIL treatment.In investigating ways to overcome this resistance,we evaluated the inhibitory effect of galangin on TRAIL resistant A549 human lung adenocarcinoma cells.Here we report that,in comparison with the single agents,the combination of galangin and TRAIL markedly suppressed proliferation of A549 cells and induced apoptosis as shown by DAPI and JC-1 staining.The combination of galangin and TRAIL induced PARP cleavage,activation of caspase-8 and p38 MAPK(mitogen-activated protein kinases).These findings indicate that the combination of galangin and TRAIL may constitute a promising strategy for the treatment of lung cancer.

关 键 词：GALANGIN TRAIL A549 cellline Cytotoxicity APOPTOSIS P38 MAPK 

分 类 号：R73[医药卫生—肿瘤]