玫瑰色考克氏菌SDB9生长素合成基因挖掘与分析  

Gene Mining and Analysis of Auxin Biosynthesis Related Genes from Kocuria Rosea SDB9

作　　者：刘畅 王建武[1] 艾银婷[1] 相微微[1] LIU Chang;WANG Jian-wu;AI Yin-ting;XIANG Wei-wei(School of Life Sciences, Yulin University, Yulin 719000, China)

机构地区：[1]榆林学院生命科学学院,陕西榆林719000

出　　处：《榆林学院学报》2021年第4期21-27,共7页Journal of Yulin University

基　　金：国家自然科学基金项目(32060027);2020年度榆林学院研究生创新基金项目(2020YLYCX07)。

摘　　要：多种植物根际促生细菌均能合成生长素,生长素在介导微生物与植物互作过程中发挥重要作用,但其合成过程多样,关于生长素的合成机理仍有待进一步明确。本研究基于玫瑰色考克氏菌SDB9的基因组数据,对生长素合成相关基因进行了挖掘与分析,发现该菌体内存2个酰胺酶基因,22个氨基转移酶基因,4个乙醛脱氢酶基因,以及色氨酸操纵子,COG分析发现trpA/gene2169具有合成吲哚的能力,KrALDH3具有依赖NAD(P)+的乙醛脱氢酶的完整保守结构域,上述基因都是合成生长素的关键基因,因此,推测SDB9菌体内同时存在色氨酸依赖和非色氨酸依赖的生长素合成途径。研究结果为进一步研究SDB9菌的生长素合成机理提供了候选基因,为深入研究其高水平合成生长素的分子机理打下基础。Auxin can be synthesized by a variety of plant growth promoting rhizobacteria.Auxin plays an important role in mediating the interaction between microorganisms and plants,but its synthesis process is diverse.The mechanism of auxin synthesis remains to be further clarified.Based on the genomic data of Kocuria rosea SDB9,the auxin synthesis related genes are mined and analyzed.It is found that there are 2 amidase genes,22 aminotransferase genes,4 aldehyde dehydrogenase genes and tryptophan operon in this strain.COG analysis shows that trpA/gene2169 has the ability to synthesize indole,and kraldh3 has NAD(P)+dependent aldehyde dehydrogenase.Therefore,it is speculated that both tryptophan dependent and non tryptophan dependent auxin biosynthesis pathways exist in SDB9.The results provide candidate genes for further study on auxin synthesis mechanism of SDB9,and lay a foundation for further study on the molecular mechanism of high-level auxin synthesis.

关 键 词：生长素 植物根际促生细菌 色氨酸 乙醛脱氢酶 

分 类 号：S144[农业科学—肥料学]