In vitro Pharmacological Activity of Chromenes in Disk of Helianthus annuus  

作　　者：Zhiying WEI Li LI Yaohua LI Zhenzhen PAN Fangchan LI Yupin CAO Jiagang DENG Xiaojiao PAN 

机构地区：[1]Teaching Center of Experiment and Practical Training, Guangxi University of Chinese Medicine, Nanning 530200, China [2]College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China [3]Guangxi Collaborative Innovation Center of Study on Functional Ingredients of Agricultural Residues, Nanning 530200, China

出　　处：《Medicinal Plant》2021年第3期50-54,共5页药用植物：英文版

基　　金：Natural Science Foundation of Guangxi(General Program No.2020GXNSFAA238033);Guangxi Collaborative Innovation Center of Study on Functional Ingredients of Agricultural Residues(CICAR2019-P4);Guangxi Key Laboratory of Pharmacodynamics Research of Traditional Chinese Medicine(19-050-39);Key Laboratory of Chinese Medicine Extraction,Purification and Quality Analysis in Guangxi Universities(Gui Jiao Ke Yan[2014]6).

摘　　要：[Objectives]The anti-tumor,anti-bacterial,anti-acetylcholinesterase and anti-α-glucosidase activity in vitro of five chromenes isolated from Helianthus annuus disk was studied,in order to provide reference for the development and utilization of H.annuus disk resources.[Methods]The effect of different concentrations of chromenes on the survival rate of leukemia HL-60 cells,lung cancer A549 cells,liver cancer SMMC-7721 cells,breast cancer MCF-7 cells and colon cancer SW480 cells was detected by MTS method,and the IC50 was calculated.The inhibitory activity of chromenes against Escherichia coli,Staphylococcus aureus subsp.aureus,Salmonella enterica subsp.enterica,Pseudomonas aeruginosa and Candida albicans was detected by microdilution method.The DTNB substrate method was used to detect the inhibitory activity of chromenes on acetylcholine.The PNPG substrate method was used to detect the inhibitory activity of chromenes onα-glucosidase.[Results]The five chromenes had no obvious in vitro inhibitory activity on the five kinds of tumor cells,with IC50 greater than 40μM.The five chromenes had no obvious in vitro inhibitory activity against the four kinds of bacteria and C.albicans.The five chromenes had certain inhibitory activity on acetylcholinesterase,and among them,6-acetyl-2,2-dimethylchromene and 6-acetyl-7-hydroxy-2,3-dimethylchromene showed strong inhibitory activity on acetylcholinesterase,with IC50 of 28.253 and 16.945μM,respectively,both smaller than that(0.275μM)of the positive control tacrine(P<0.01).The five chromenes showed good inhibitory effect onα-glucosidase,and among them,7-hydroxy-6-hydroxyacetyl-2,2-dimethylchromene and 6-acetyl-7-hydroxy-2,3-dimethylchromene had stronger inhibitory activity,with IC50 of 20.240 and 21.052μM,respectively,significantly better than that(169.780μM)of the positive control acarbose(P<0.01).[Conclusions]The five chromenes in H.annuus disk have certain in vitro inhibitory activity against acetylcholinesterase andα-glucosidase and certain potential in fighting neur

关 键 词：Helianthus annuus disk CHROMENE Pharmacological activity ACETYLCHOLINESTERASE Α-GLUCOSIDASE 

分 类 号：R73[医药卫生—肿瘤]