Effect of transcutaneous auricular vagus nerve stimulation on fasting blood glucose and serum insulin concentration in Zucker diabetes fatty rats  

经皮耳迷走神经刺激对Zucker糖尿病大鼠血糖及血清胰岛素浓度的影响

作　　者：Zi-xuan ZHANG Shao-yuan LI Yu WANG Yue ZHANG Yi-fei WANG Pei-jing RONG 张紫璇;李少源;王瑜;张悦;王艺霏;荣培晶(中国中医科学院针灸研究所,北京100700)

机构地区：[1]Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China

出　　处：《World Journal of Acupuncture-Moxibustion》2021年第3期212-217,共6页世界针灸杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China:81674072;Sino-German Cooperative Research Project of National Natural Science Foundation of China:GZ1236;National Natural Science Foundation of China Youth Project:82004181。

摘　　要：Objective:To observe the influence of transcutaneous auricular vagus nerve stimulation(taVNS) on fasting plasma glucose(FPG) and serum insulin(INS) in Zucker diabetes fatty(ZDF) rats and explore the regulatory effect of taVNS on blood glucose in ZDF rats.Methods:A total of 20 male ZDF rats were randomized into a model group and a taVNS group,10 rats in each one.Besides,the other 10 Zucker Lean(ZL) rats were selected to be a control group.The rats in the control group were fed with common forage and those in the model group and the taVNS group were fed with high-sugar and high-fat forage.The intervention of electric stimulation was applied in the rats of taVNS group,with 2/15 Hz in frequency,disperse-dense wave and 2 mA in intensity.Each intervention lasted 30 min,once a day,consecutively for 12 weeks.No any intervention was given in the control group and the model group.The body mass and FPG level were recorded once every 2 weeks in the rats.After the experiment,the rats were sacrificed and blood sample was collected.Enzyme linked immunosorbent assay(ELISA) was adopted to determine the level of serum INS in the rats.Results:After the modeling,compared with the control group,the body mass and FPG level were all higher(P <0.05,P <0.01) and the concentration of serum INS was lower(P <0.01) in the rats of the model group.After intervention,compared with the model group,the body mass was lower in week 6 to 10(P <0.01,P <0.05),FPG level was lower in week 8 to 12(P <0.01,P <0.05) and serum INS concentration was higher(P <0.01) in the rats of the taVNS group.Conclusion:TaVNS apparently improves in hyperglycemia in ZDF rats and increases serum insulin concentration in the rats.

关 键 词：Transcutaneous auricular vagus nerve stimulation Type 2 diabetes mellitus Zucker diabetic fatty rat INSULIN HYPOGLYCEMIC 

分 类 号：R587.1[医药卫生—内分泌]