宫颈癌患者外周血记忆T细胞的分布特征及临床意义  

Distribution and clinical significance of memory T cells in peripheral blood of patients with cervical cancer

作　　者：杨国臣 经莉 陆晓媛 YANG Guo-chen;JING Li;LU Xiao-yuan(Xuzhou Medical University,Xuzhou,Jiangsu 221000,China;不详)

机构地区：[1]徐州医科大学,江苏徐州221000 [2]徐州医科大学附属医院,江苏徐州221000

出　　处：《中国临床研究》2021年第7期895-899,共5页Chinese Journal of Clinical Research

摘　　要：目的探讨宫颈癌患者外周血中CD4^(+)初始T细胞(Tn)、记忆T细胞(Tm)与CD8CD4^(+)Tn及Tm的分布特点及临床价值。方法采用横断面研究的方法选取2020年1月至5月徐州医科大学附属医院住院的宫颈癌Ⅰ、Ⅱ期患者30例为观察组,同期15例宫颈炎患者作为对照组。采集外周静脉血2 ml,流式细胞学检测外周血CD4CD4^(+)Tn、Tm与CD8CD4^(+)Tn、Tm的比例。结果与对照组患者相比,观察组外周血中CD4CD4^(+)Tn比例差异无统计学意义(t=1.96,P=0.06),CD8CD4^(+)Tn的比例显著降低(t=6.57,P<0.01),CD4CD4^(+)Tm、CD8CD4^(+)Tm、CD4CD4^(+)Tm/Tn和CD8CD4^(+)Tm/Tn的比例则明显上升(P<0.05,P<0.01)。观察组外周血中CD4CD4^(+)Tn、CD8CD4^(+)Tn比例与淋巴结转移负相关,而CD4CD4^(+)Tm、CD4CD4^(+)Tm/Tn、CD8CD4^(+)Tm/Tn比例与淋巴结转移正相关,CD8CD4^(+)Tm、CD8CD4^(+)Tm/Tn与临床分期负相关,差异均有统计学意义(P<0.05,P<0.01)。结论在早期宫颈癌发展的过程中,机体的抗肿瘤免疫储备能力逐渐下降,但是机体通过增强免疫细胞向记忆细胞的转化,增强Tm细胞免疫效能,从而提高机体抗肿瘤的免疫效应。Objective To investigate the distribution and c linical value of CD4^(+)naive T cells(Tn),memory T cells(Tm),and CD8^(+)Tn and Tm in peripheral blood of patients with cervical cancer.Methods By a cross-sectional study between January 2020 and May 2020,30 patients with stage Ⅰ and Ⅱ cervical cancer in the Affiliated Hospital of Xuzhou Medical University were selected as observation group and 15 patients with cervicitis were served as control group.After the peripheral venous blood(2 ml)samples were collected,the levels of CD4^(+)Tn,CD4^(+)Tm and CD8^(+)Tn,CD8^(+)Tm were detected by flow cytometry.Results There was no significant difference in the level of CD4^(+)Tn between two groups(t=1.96,P=0.06).Compared with control group,the level of CD8^(+)Tn decreased(t=6.57,P<0.01),and the levels of CD4^(+)Tm,CD8^(+)Tm,CD4^(+)Tm/Tn and CD8^(+)Tm/Tn increased significantly(P<0.05,P<0.01).The levels of CD4^(+)Tn and CD8^(+)Tn were negatively correlated with lymph node metastasis,while the levels of CD4^(+)Tm,CD4^(+)Tm/Tn and CD8^(+)Tm/Tn were positively correlated with lymph node metastasis,and the levels of CD8^(+)Tm and CD8^(+)Tm/Tn were negatively correlated with clinical stage(P<0.05,P<0.01).Conclusion In early cervical cancer development,the antitumor immune reserve capacity gradually decreases,but the transformation of immune cells into memory T cells can enhance its immune efficiency so as to improve the anti-tumor immune response in the patients.

关 键 词：宫颈癌 初始T细胞 记忆T细胞 T淋巴细胞亚群 

分 类 号：R737.33[医药卫生—肿瘤] R446.6[医药卫生—临床医学]