深海真菌Neoroussoella sp.固体发酵物中次生代谢产物及其生物活性研究  

Study on the secondary metabolites from the deep-sea-derived fungus Neoroussoella sp.and their biological activities

作　　者：钟晋谦 陈玉婵 陈闪冲 刘昭明 刘洪新 章卫民 严寒静 ZHONG Jin-qian;CHEN Yu-chan;CHEN Shan-chong;LIU Zhao-ming;LIU Hong-xin;ZHANG Wei-min;YAN Han-jing(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,State Key Laboratory of Applied Microbiology Southern China,Institute of Microbiology,Guangdong Academy of Sciences, Guangzhou 510070,China)

机构地区：[1]广东药科大学中药学院,广州510006 [2]广东省科学院微生物研究所,华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室,广州510070

出　　处：《天然产物研究与开发》2021年第7期1165-1171,共7页Natural Product Research and Development

基　　金：广东省海洋经济发展专项([2020]042);广东省自然科学基金团队项目(2016A030312014);国家自然科学基金(41906106);广东省特支计划(2019TQ05Y375)。

摘　　要：研究深海真菌Neoroussoella sp.FS526的次生代谢产物及生物活性。采用硅胶柱、Sephadex LH-20凝胶柱和高效液相等多种色谱技术对菌株FS526的固体发酵物中的次生代谢产物进行分离纯化,并通过NMR等波谱方法鉴定化合物结构。从菌株FS526中共分离鉴定了15个化合物,分别为amestolkolide B(1)、thailandolide B(2)、aspergillumarins A和B(3和4)、de-O-methyldiaporthin(5)、4-(2-acetoxyethyl)phenol(6)、5,6,8-三羟基-4-(1′-羟乙基)异香豆素(7)、sescandelin(8)、penicifuran A(9)、7-羟基-5-甲氧基-4,6-邻苯二甲酯(10)、pestalotiolactone A(11)、2′,4′-二羟基-6′-甲氧基-3′-甲基苯乙酮(12)、1-(2,4-二羟基-6-甲氧基-3-甲基苯基)乙酮(13)、2,5-二甲基间苯二酚(14)、3,5-二羟基甲苯(15)。所有化合物均为首次从该属真菌中分离得到。细胞毒和抗氧化活性测试表明化合物1具有较弱的细胞毒活性,其IC50值为41.44~77.89μM;化合物7具有较强的抗氧化活性,其IC50值为13.93μM。The secondary metabolites of a deep-sea-derived fungus Neoroussoella sp.FS526 and their biological activities were investigated.The solid fermentation extraction of strain FS526 was isolated and purified by silica gel column,Sephadex LH-20 gel column,and high performance liquid chromatography,which led to the isolation of fifteen known compounds.The chemical structures of the isolates were identified by spectrum analysis as amestolkolide B(1),thailandolide B(2),aspergillumarins A-B(3 and 4),de-O-methyldiaporthin(5),4-(2-acetoxyethyl)phenol(6),5,6,8-trihydroxy-4-(1′-hydroxyethyl)isocoumarin(7),sescandelin(8),penicifuran A(9),7-hydroxy-5-methoxy-4,6-dimethylphthalidc(10),pestalotiolactone A(11),2′,4′-dihydroxy-6′-methoxy-3′-methylacetophenone(12),1-(2,4-dihydroxy-6-methoxy-3-methylphenyl)ethanone(13),2,5-dimethylresorcinol(14),and 3,5-dihydroxytoluene(15).All the isolated compounds were firstly reported from the genus Neoroussoella.Compound 1 showed weak cytotoxic activity with the IC50 values of 41.44-77.89μM,and compound 7 showed strong antioxidant activity with the IC50 value of 13.93μM.

关 键 词：深海真菌 Neoroussoella sp. 次生代谢产物 细胞毒活性 抗氧化活性 

分 类 号：R914.4[医药卫生—药物化学]