高压脉冲电场预处理普洱熟茶对SD大鼠体内活性氧(ROS)水平的影响  

Effect of Pre-treatment of Pu’er Tea by High Voltage Pulse Electric Field on Reactive Oxygen(ROS)Levels in SD Mice

作　　者：冷彦 王静[1,2,3] 吴文斗 沈晓静[1,2,3] 陈立佼 杨贺凯[1,2,3] 吴奇 王白娟[1,2,3] LENG Yan;WANG Jing;WU Wen-dou;SHEN Xiao-jing;CHEN Li-jiao;YANG He-kai;WU Qi;WANG Bai-juan(Yunnan Agricultural University,Yunnan Kunming 650201,China;Yunnan Organic Tea Industry Intelligent Engineering Research Center,Yunnan Kunming 650201,China;Key Laboratory of Intelligent Organic Tea Garden in Yunnan Province,Yunnan Kunming 650201,China)

机构地区：[1]云南农业大学,云南昆明650201 [2]云南省有机茶产业智能工程研究中心,云南昆明650201 [3]云南省高校智能有机茶园建设重点实验室,云南昆明650201

出　　处：《西南农业学报》2021年第6期1208-1214,共7页Southwest China Journal of Agricultural Sciences

基　　金：中央引导地方科技发展专项资金“云南有机普洱茶数字茶园科技创新建设基地”(202007AB110001);云南省科技厅重大科技专项子课题“‘云茶’数字化关键技术应用研发与示范”(202002AE09001004);云南省市场监督管理局知识产权现代服务体系建设项目“云南普洱茶产业知识产权战略联盟建设”(201902);云南省万人计划“产业技术领军人才”专项;云南省教育厅科学研究基金“基于机器视觉的勐海大叶茶生长监测系统研究”(2020Y0171)。

摘　　要：【目的】分析不同高压脉冲电场(high voltage pulsed electric field, HPEF)预处理普洱熟茶对SD大鼠体内活性氧(reactive oxygen species, ROS)的影响。【方法】采用DCFH-DA(2,7-dichlorofuorescin diacetate)结合多功能荧光酶标仪检测大鼠肝、肾、心、脑组织内ROS水平的变化,并利用数据分析软件进行统计分析。【结果】与空白对照相比,HPEF预处理组与未预处理组均能降低大鼠肝、肾、心、脑组织内ROS水平,剂量越高ROS水平越低,且除心脏外,其余组织HPEF预处理组对ROS的抑制作用优于未预处理组;HPEF预处理高剂量组(2.0 g·kg^(-1)·bw)处理8周后,肝脏、肾脏和脑组织中ROS水平可分别降低24.51%、63.20%、68.24%,显著高于空白组(P<0.01);中剂量组(1.0 g·kg^(-1)·bw)可以降低肝脏、肾脏和脑组织中ROS水平20.67%、60.41%、64.17%,显著高于空白组(P<0.01);低剂量组(0.5 g·kg^(-1)·bw)可以降低肾脏和脑组织中ROS水平55.91%、57.67%,明显高于空白组(P<0.01)。除空白组外,大鼠肝、肾、心、脑组织内ROS水平随时间的增加而降低。【结论】普洱熟茶可降低大鼠肝、肾、心、脑组织内的ROS水平,HPEF预处理可提高普洱茶的该抑制作用,可为普洱茶功效研究提供新思路。【Objective】The effects of different high voltage pulse electric field(HPEF)pretreatment Pu’er ripe tea on reactive oxygen species(ROS)in SD mice were analyzed.【Method】The changes of the levels of ROS in the liver,kidney,heart and brain of mice were detected by means of DCFH-DA(2,7-dichlorum diacetate),and the data analysis software was used for statistical analysis.【Result】Compared with the blank group,the levels of ROS in mice liver,kidney,heart and brain tissue could be reduced in Pre-treatments group and Un-treatments group,and the higher the dose was,the lower the level of ROS was,and with the exception of the heart,the inhibitory effect of the other tissues Pre-treatments group on the ROS was better than that of the Un-treatments group.With the higher dose(2.0 g·kg^(-1)·bw)of HPEF pretreatment 8 weeks,the levels of ROS in liver,kidney and brain tissues decreased by 24.51%,63.20%,68.24%,which were significantly higher than those in the blank group(P<0.01);With the medium dose group(1.0 g·kg^(-1)·bw),the levels of ROS in liver,kidney and brain reduced by 20.67%,60.41%,64.17%,which were significantly higher than those in the blank group(P<0.01);with the low dose group(0.5 g·kg^(-1)·bw),the levels of ROS in kidney and brain could reduce by 55.91%,57.67%,which were significantly higher than those in the blank group(P<0.01).Except the blank group,the ROS in liver,kidney,heart and brain decreased with time.【Conclusion】Pu’er ripe tea could reduce the ROS levels in liver,kidney,heart and brain of mice,and HPEF Pre-treatments could improve the inhibitory effect of Pu’er ripe tea,which could provide a new idea for the study of the efficacy of Pu’er ripe tea.

关 键 词：高压脉冲电场 普洱熟茶 DCFH-DA 活性氧 

分 类 号：S571[农业科学—作物学]