葡萄多酚改善高脂饮食诱导的小鼠高血脂及对肠道菌群的调节作用  

Grape Polyphenol Attenuated Hyperlipidemia and Modulated Gut Microbiota in High-fat Diet-fed Mice

作　　者：卢烽 刘凤娇[1,2] 胡小松 张燕[1,2] Lu Feng;Liu Fengjiao;Hu Xiaosong;Zhang Yan(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083;National Engineering Research Centre for Fruit and Vegetable Processing,Beijing 100083)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]国家果蔬加工工程技术研究中心,北京100083

出　　处：《中国食品学报》2021年第7期97-106,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2017YFD0400500)。

摘　　要：以C57BL/6雄性小鼠建立高血脂模型,研究葡萄多酚干预下小鼠的体重、血脂等指标的变化,应用16S RNA高通量测序技术检测了多酚干预下高血脂小鼠肠道菌群的变化。结果表明:葡萄皮渣多酚提取物(GPE)和葡萄原花青素(GSE)能显著抑制高脂饮食C57BL/6J小鼠的体重增加。GPE灌胃8周能显著降低LDL-c水平;GSE则可显著降低TG、TC、LDL-c水平,表明GPE和GSE能改善高脂饮食小鼠的脂质紊乱并表现出降血脂,改善肝脏脂质累积的效果。通过分析多酚干预下高脂饮食小鼠肠道菌群的变化,发现GPE、GSE具有调节高脂饮食介导的肠道菌群紊乱的功能,并且这可能与其改善脂质代谢的作用相关。In this experiment,C57BL/6 male mice were used to study the effects of grape polyphenol complex on blood lipid reduction based on the changes in body weight and blood lipids in the condition of high-fat diet.The 16S RNA analysis was used to explore the gut microbiota.It was found that grape pomace polyphenols extracts(GPE)and grape seed polyphenols extracts(GSE)could significantly inhibit the weight gain of high-fat diet C57BL/6J mice.GPE gavage for 8 weeks could significantly reduce LDL-c level,while GSE could significantly reduce TG,TC,LDL-c levels.Thus,GPE and GSE could improve lipid disorders in high-fat diet mice and showed a certain effect of lowering blood lipid.Therefore,the intake of GPE and GSE is important for the prevention of hyperlipidemia.Besides,in this experiment,gut microbiota sequencing was used to explain the functional mechanism of grape polyphenol complex,and the effects of intestinal microbes and polyphenols on blood lipids were analyzed.It was found that the gut microbiota affected lipid metabolism and participated in the adjustment of GPE and GSE on blood lipids levels.

关 键 词：葡萄多酚 高血脂 肠道菌群 

分 类 号：R589.2[医药卫生—内分泌] R-332[医药卫生—内科学] TS201.4[医药卫生—临床医学]