Tumor formation of prostate cancer cells influenced by stromal cells from the transitional or peripheral zones of the normal prostate  

作　　者：Fu-Jun Zhao Bang-Min Han Sheng-Qiang Yu Shu-Jie Xia 

机构地区：[1]Department of Urology,Shanghai First People’s Hospital,Shanghai Jiao Tong University,Shanghai 200080,China

出　　处：《Asian Journal of Andrology》2009年第2期176-182,共7页亚洲男性学杂志（英文版）

基　　金：the National Natural Science Foundation of China(No.30672109).

摘　　要：This study was designed to investigate the different involvements of prostatic stromal cells from the normal transitional zone(TZ)or peripheral zone(PZ)in the carcinogenesis of prostate cancer(PCa)epithelial cells(PC-3)in vitro and in vivo co-culture models.Ultra-structures and gene expression profiles of primary cultures of human prostatic stromal cells from the normal TZ or PZ were analyzed by electron microscopy and microarray analysis.In vitro and in vivo co-culture models composed of normal TZ or PZ stromal cells and human PCa PC-3 cells were established.We assessed tumor growth and weight in the in vivo nude mice model.There are morphological and ultra-structural differences in stromal cells from TZ and PZ of the normal prostate.In all,514 differentially expressed genes were selected by microarray analysis;483 genes were more highly expressed in stromal cells from TZ and 31 were more highly expressed in those from PZ.Co-culture with PZ stromal cells and transforming growth factor-β1(TGF-β1)increased the tumor growth of PC-3 cells in vitro and in vivo,as well as Bcl-2 expression.On the other hand,stromal cells of TZ suppressed PC-3 cell tumor growth in the mouse model.We conclude that ultra-structures and gene expression differ between the stromal cells from TZ or PZ of the normal prostate,and stroma-epithelium interactions from TZ or PZ might be responsible for the distinct zonal localization of prostate tumor formation.

关 键 词：CO-CULTURE epithelial cells stromal cells transitional zone peripheral zone prostate cancer 

分 类 号：R73[医药卫生—肿瘤]