蒲公英根不同多糖组分的降血糖作用及调控途径研究  

Hypoglycemic effect and regulatory pathway of different polysaccharide components from dandelion root

作　　者：吴亚楠 邹辉[1] 刘玉茜 陈义伦[1] WU Yanan;ZOU Hui;LIU Yuqian;CHEN Yilun(College of Food Science and Engineering,Shandong Agricultural University,Taian 271000,China)

机构地区：[1]山东农业大学食品科学与工程学院,山东泰安271000

出　　处：《食品与发酵工业》2021年第15期90-97,共8页Food and Fermentation Industries

基　　金：山东省农业重大应用技术创新项目(2130106)。

摘　　要：以蒲公英根粗多糖(dandelion root polysaccharide,DP)经乙醇分级沉淀层析洗脱后制得DP1、DP2、DP3这3种组分为材料,利用细胞和动物实验,探究蒲公英根多糖的降血糖活性,确定活性多糖组分,并初步分析了其单糖组成及降糖途径。其中DP2显示出最好的降血糖效果。3种多糖均能提高糖尿病小鼠的葡萄糖耐受力,且DP2的改善效果最佳。多糖干预组中甘油三酯、总胆固醇和低密度脂蛋白胆固醇含量与模型组相比显著降低,高密度脂蛋白胆固醇含量明显升高,说明3种组分均可以改善血脂异常。此外,3种蒲公英根多糖对肝脏具有一定的保护作用。对于2型糖尿病模型小鼠,DP1的肝糖原含量最多,对于人肝癌细胞胰岛素抵抗模型,DP2葡萄糖消耗量最多,DP3对小鼠胰岛素瘤β细胞胰岛损伤模型的保护作用最佳。最后可推测3种蒲公英根多糖的分子结构的差异,使其通过调控不同的降糖途径而具有不同的降血糖效果。DP1,DP2 and DP3 were prepared from crude polysaccharide by ethanol fractional precipitation chromatography.The hypoglycemic activity of polysaccharides from dandelion root was studied by cell and animal experiments.The results showed that DP2 had the best hypoglycemic effect.All the three polysaccharides could improve the glucose tolerance of diabetic mice,and DP2 had the best effect.Compared with the model group,the contents of TC,TG and LDL-C in the polysaccharide intervention group were significantly decreased,while the content of HDL-C was significantly increased which indicating that the three components had benefit on dyslipidemia.In addition,dandelion root polysaccharide had a certain protective effect on the liver.The liver glycogen content of DP1 was the highest in type 2 diabetic mice and the glucose consumption of DP2 was the highest in HepG2 insulin resistance model.While,DP3 showed obvious protective effect on damaged islet cells.Based on these results,it could speculate that the molecular structure of the three kinds of dandelion root polysaccharides was different,which makes them have different hypoglycemic effects.

关 键 词：蒲公英 纯化 多糖 降血糖 作用途径 

分 类 号：R285.5[医药卫生—中药学]