偏重亚硫酸钠对微生物法测定维生素B_(12)含量的影响  

Influence of sodium metabisulfite on the determination of vitamin B_(12) by microbiological method

作　　者：杨亚芳 李倩 刘凯 郭晶晶 毕思丹 杨文炼 李爽 王雨婷 巴冬梅 蔡雪凤 YANG Ya-Fang;LI Qian;LIU Kai;GUO Jing-Jing;BI Si-Dan;YANG Wen-Lian;LI Shuang;WANG Yu-Ting;BA Dong-Mei;CAI Xue-Feng(Beijing Food Safety Monitoring and Risk Assessment Center(Beijing Food Inspection Institute),National Food Quality and Safety Inspection and Supervision Center,Beijing 100094,China)

机构地区：[1]北京市食品安全监控和风险评估中心(北京市食品检验所),国家食品质量安全检验监督中心,北京100094

出　　处：《食品安全质量检测学报》2021年第11期4660-4665,共6页Journal of Food Safety and Quality

基　　金：首都食品质量安全保障基金项目(Z191100008619007)。

摘　　要：目的探究偏重亚硫酸钠对微生物法测定婴幼儿食品和乳品中维生素B_(12)(vitaminB_(12),VB_(12))含量的影响。方法通过在标准工作液和样品待测液中添加质量浓度梯度为0、0.0025、0.005、0.02、0.08和0.16 mg/mL偏重亚硫酸钠,测定不同处理组实验样品中VB_(12)含量值并对质控样品进行质控结果分析,研究偏重亚硫酸钠对莱士曼氏乳杆菌生长的促进作用及其对原生VB_(12)提取的影响。结果在标准溶液中添加质量浓度为0~0.16 mg/mL的偏重亚硫酸钠都可以促进莱士曼氏乳杆菌的生长。在0~0.05 mg/mL的范围内时,偏重亚硫酸钠能促进样品中原生VB_(12)的提取,且随偏重亚硫酸钠质量浓度增加,原生VB_(12)的提取会成比例增加。结论偏重亚硫酸钠在0~0.16 mg/mL浓度范围内可以促进莱士曼氏乳杆菌的生长,提高食品原生VB_(12)的提取率,进而增加检测结果的稳定性和准确度。Objective To explore the effect of sodium metabisulfite on the determination of vitamin B_(12)(VB_(12))in infant food and dairy products by microbiological method.Methods By adding mass concentration gradients of 0,0.0025,0.005,0.02,0.08 and 0.16 mg/mL sodium metabisulfite to the standard working solution and the sample solution,the content of VB_(12)in the experimental samples of different treatment groups was detected and the results of quality control samples were analyzed,the promotion node of sodium metabisulfite on Lactobacillus leichmannii and its effect on the extraction of the original form of VB_(12)were explored.Results Adding sodium metabisulfite with a mass concentration of 0‒0.16 mg/mL to the standard solution could promote the growth of Lactobacillus leichmannii.Sodium metabisulfite could promote the extraction of native VB_(12)in the samples in the range of 0‒0.05 mg/mL,and as the concentration of sodium metabisulfite increases,the extraction of native VB_(12)would be proportionally increased.Conclusion In the concentration range of 0‒0.16 mg/mL,sodium metabisulfite can promote the growth of Lactobacillus leichmannii,increase the extraction rate of native VB_(12),and thereby increase the stability and accuracy of the test results.

关 键 词：维生素B_(12) 微生物法 偏重亚硫酸钠 莱士曼氏乳杆菌 

分 类 号：TS207.3[轻工技术与工程—食品科学]