瓜拉纳化学成分的UPLC-Q-TOF-MS分析  

Analysis of Chemical Constituents in Paullinia cupana Dried Seeds by UPLC-Q-TOF-MS

作　　者：翁倩倩 杨滨[2] 李斌[1] 邓爱平[2] 赵佳琛 林王敏 詹志来 黄璐琦[2] WENG Qian-qian;YANG Bin;LI Bin;DENG Ai-ping;ZHAO Jia-chen;LIN Wang-min;ZHAN Zhi-lai;HUANG Lu-qi(Academician Workstation of Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;College of Pharmaceutical Science,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]江西中医药大学院士工作站,南昌330004 [2]中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京100700 [3]浙江中医药大学药学院,杭州310053

出　　处：《中国实验方剂学杂志》2021年第15期68-75,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中央本级重大增减支项目(2060302);中国中医科学院基本科研业务费项目(ZZ13-YQ-091);国家重点研发计划项目(2019YFC1711401,2017YFC1700805)。

摘　　要：目的:对瓜拉纳中的化学成分进行系统分析,为该植物的深入研究及开发利用提供依据。方法:参照国家标准及相关文献对瓜拉纳中所含的粗蛋白、粗脂肪、粗多糖、粗纤维等大分子成分进行含量测定,应用超高效液相色谱-四极杆-飞行时间串联质谱法(UPLC-Q-TOF-MS)对瓜拉纳的化学成分进行系统定性分析,采用ACQUITY UPLC-HSS-T3色谱柱(2.1 mm×100 mm,1.8μm),以0.1%甲酸水溶液(A)-0.1%甲酸乙腈溶液(B)为流动相进行梯度洗脱(0~5 min,2%~10%B;5~6 min,10%~20%B;6~9 min,20%~30%B;9~9.5 min,30%~35%B;9.5~10.5 min,35%~45%B;10.5~13 min,45%~55%B;13~15 min,55%~80%B;15~19 min,80%~98%B;19~20 min,98%B;20~20.3 min,98%~2%B;20.3~23 min,2%B),电喷雾离子源(ESI),正、负离子模式进行检测,扫描范围m/z 50~1 500,根据精确相对分子质量及碎片信息,结合数据库匹配和对照品比对进行结构鉴定。结果:瓜拉纳中粗蛋白、粗脂肪、粗多糖及粗纤维质量分数分别为(0.63±0.03)%,(2.73±0.09)%,(3.23±0.12)%,(8.89±0.59)%。通过UPLC-Q-TOF-MS分析从瓜拉纳中共鉴定出42个成分,包括3个甲基黄嘌呤类成分,2个核苷类成分,1个氨基酸类成分,3个有机酸,33个黄酮类成分,其中3个化合物(L-色氨酸、表没食子儿茶素没食子酸酯、大豆苷元)是从瓜拉纳中首次发现。结论:瓜拉纳中的营养成分含量丰富,作为功能食品来开发具有良好潜力。UPLC-Q-TOF-MS技术为鉴别瓜拉纳药材中化学成分提供简便、快速、准确的方法,其主要化学成分为甲基黄嘌呤类、原花青素类成分,可为瓜拉纳药材的质量评价及药效物质基础研究提供参考。Objective: The chemical constituents in guarana(Paullinia cupana dried seeds)were systematically analyzed to provide a basis for further research, development and utilization of this plant.Method: The contents of crude protein,crude fat,crude polysaccharide and crude fiber in guarana were determined according to national standards and related documents,and the chemical constituents of guarana was qualitatively analyzed by ultra performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS),ACQUITY UPLC-HSS-T3 column(2.1 mm×100 mm,1.8 μm)was used with 0.1%formic acid aqueous solution(A)-0.1% formic acid acetonitrile solution(B)as mobile phase for gradient elution(0-5 min,2%-10%B;5-6 min,10%-20%B;6-9 min,20%-30%B;9-9.5 min,30%-35%B;9.5-10.5 min,35%-45%B;10.5~13 min,45%-55%B;13-15 min,55%-80%B;15-19 min,80%-98%B;19-20 min,98%B;20-20.3 min,98%-2%B;20.3-23 min,2%B),the electrospray ionization(ESI)was used for detection in positive and negative ion modes,the scanning range was m/z 50-1 500,and the structure was identified according to the relative molecular weight and fragment information combined with database matching and comparison of reference substances. Result: The contents crude protein,crude fat,crude polysaccharide and crude fiber in guarana were(0.63±0.03)%,(2.73±0.09)%,(3.23±0.12)% and(8.89±0.59)%,respectively. A total of 42 chemical constituents in guarana were identified by UPLC-Q-TOF-MS,including 3 methylxanthines,2 nucleosides, 1 amino acid, 3 organic acids, 33 flavonoids, 3(L-tryptophan, epigallocatechin gallate,daidzein)of which were first discovered in guarana. Conclusion: Guarana is rich in nutrients and has good potential to be developed as a functional food. UPLC-Q-TOF-MS technique provides a simple,rapid and accurate method for the identification of chemical constituents in guarana. Methylxanthines and proanthocyanidins are the main chemical constituents of guarana,which is meaningful for quality evaluation and material basis of guarana.

关 键 词：瓜拉纳 化学成分 超高效液相色谱-四极杆-飞行时间串联质谱法(UPLC-Q-TOF-MS) 甲基黄嘌呤类 原花青素类 氨基酸 黄酮类 

分 类 号：R22[医药卫生—中医基础理论] R28[医药卫生—中医学]