氨基酸生产的代谢工程研究进展与发展趋势  

Advances and prospects in metabolic engineering for the production of amino acids

作　　者：马倩[1] 夏利 谭淼 孙全伟 杨蒙雅 张颖[1] 陈宁[1] Qian Ma;Li Xia;Miao Tan;Quanwei Sun;Mengya Yang;Ying Zhang;Ning Chen(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《生物工程学报》2021年第5期1677-1696,共20页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(No.2018YFA0900300);国家自然科学基金(No.21808168)资助。

摘　　要：氨基酸发酵是我国发酵工业的支柱产业,近年来,随着代谢工程的快速发展,氨基酸的代谢工程育种蓬勃发展。传统的正向代谢工程、基于组学分析与计算机模拟的反向代谢工程以及借鉴自然进化的进化代谢工程,都有越来越多的应用。在氨基酸的工业生产中涌现出了一系列具有高效生产、抗逆性强等优良性状的菌株。日益剧烈的市场竞争对菌株的选育提出了新的要求,如开发高附加值氨基酸品种、菌株代谢的动态调控、适应新工艺的要求等。文中介绍了氨基酸生产相关的代谢工程研究进展以及未来的发展趋势。Fermentative production of amino acids is one of the pillars of the fermentation industry in China.Recently,with the fast development of metabolic engineering and synthetic biology technologies,the metabolic engineering for production of amino acids has been flourishing.Conventional forward metabolic engineering,reversed metabolic engineering based on omics data and in silico simulation,and evolutionary metabolic engineering mimicking the natural evolution,have shown increasingly promising applications.A series of highly efficient and robust amino acids-producing strains have been developed and applied in the industrial production of amino acids.The increasingly fierce market competition has put forward new requirements for strain breeding and selection,such as developing high value-added amino acids,dynamic regulation of cellular metabolism,and adapting to the requirements of new process.This review summarizes the advances and prospects in metabolic engineering for the production of amino acids.

关 键 词：氨基酸 代谢工程 大肠杆菌 谷氨酸棒杆菌 生物传感器 

分 类 号：TQ922[轻工技术与工程—发酵工程]