微波辅助高压法提取金针菇黄酮的纯化及活性测定  

Purification and Activity Determination of Flavonoids Extracted from Flammulina velutipes by Microwave Assisted High Pressure Method

作　　者：王广慧[1] 于德涵[1] 黎莉[1] WANG Guanghui;YU Dehan;LI Li(College of Food and Pharmaceutical Engineering,Suihua University,Suihua,Heilongjiang 152061,China)

机构地区：[1]绥化学院食品与制药工程学院,黑龙江绥化152061

出　　处：《陕西农业科学》2021年第7期75-79,90,共6页Shaanxi Journal of Agricultural Sciences

基　　金：黑龙江省省属高等学校基本科研业务费基础研究项目(YWK10236200203);国家自然科学基金项目(21201128)。

摘　　要：为了确定微波辅助高压法所制备的金针菇黄酮的生物活性,及其经H103型大孔树脂纯化的最优工艺条件,对此纯化工艺进行了优化,并检测了黄酮的抗菌性,以及它清除2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(ABTS·)、1,1-二苯基-2-三硝基苯肼自由基(DPPH·)和羟基自由基(OH·)的活性。结果显示,此纯化工艺的最优条件是吸附时,样品液pH值为5,浓度为1.45 mg·mL^(-1),流速为0.30 mL·min^(-1);洗脱时,溶剂乙醇的浓度为70%,流速为2 mL·min^(-1),用量为60 mL,纯化后黄酮纯度可达纯化前的3.27倍。金针菇黄酮对四种菌株皆表现出抗菌性,其中对大肠杆菌(Escherichia coli)的抗菌活性最强,其次是沙门氏菌(Salmonella enteritidis),金黄色葡萄球菌(Staphylococcus aureus)第三,枯草芽孢杆菌(Bacillus subtilis)最弱。该黄酮可清除三种自由基,但清除率都低于VC。可见微波辅助高压法所制备的金针菇黄酮兼具抗菌和抗氧化能力,H103型大孔树脂可以用作纯化金针菇黄酮的介质。In order to determine the biological activity of flavonoids from Flammulina velutipes by microwave-assisted high-pressure method and to find its optimal process conditions for purification by H103 macroporous resin,the purification process was optimized and the purified flavonoids were tested for their antibacterial ability and removal activity of 2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diammonium salt free radicals(ABTS·),1,1-diphenyl-2-trinitrophenylhydrazine free radicals(DPPH·)and hydroxyl free radicals(OH-)in this paper.The optimal conditions for this purification process were that the pH of the sample solution was 5,the concentration was 1.45 mg·mL^(-1),and the flow rate was 0.30 mL·min^(-1) during adsorption;the concentration of the solvent ethanol was 70%,the flow rate was 2 mL·min^(-1),the dosage was 60 mL during eluting.The purity of flavonoids was 3.27 times higher than that before purification.Flavonoids of Flammulina velutipes showed antibacterial activity against all the four strains,of which the antibacterial activity against Escherichia coli was the strongest,followed by Salmonella enteritidis,Staphylococcus aureus was in the third place,and Bacillus subtilis was the weakest.Flammulina flavonoids could scavenge three free radicals,but the scavenging rate was lower than VC.So Flammulina flavonoids prepared by microwave-assisted high-pressure method has both antibacterial and antioxidative capabilities.H103 macroporous resin can be used as a medium for purifying Flammulina flavonoids.

关 键 词：微波辅助高压法 金针菇黄酮 大孔树脂 抗菌性 抗氧化性 

分 类 号：TS[轻工技术与工程]