Transcriptomic analysis of interstockinduced dwarfism in Sweet Persimmon(Diospyros kaki Thunb.)  

作　　者：Yanying Shen Weibing Zhuang Xutong Tu Zhihong Gao Aisheng Xiong Xinyi Yu Xuehan Li Feihong Li Shenchun Qu 

机构地区：[1]College of Horticulture,Nanjing Agricultural University,210095 Nanjing,Jiangsu,China [2]Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,210014 Nanjing,China [3]Jiangsu Key Laboratory for the Research and Utilization of Plant Resources,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,210014 Nanjing,China

出　　处：《Horticulture Research》2019年第1期1086-1102,共17页园艺研究（英文）

基　　金：supported by the Science and Technology Special Project in North Jiangsu Research Funds(SZ-LYG2017004);the National Public Welfare Industry(Agriculture)Project Special Scientific Research Funds(201203047).

摘　　要：Growth monitoring indicated that the height of‘Kanshu’plants with‘Nantong-xiaofangshi’as an interstock was significantly shorter than that of‘Kanshu’plants with no interstock.A transcriptome analysis of the two graft combinations(‘Kanshu’/Diospyros lotus and‘Kanshu’/‘Nantong-xiaofangshi’/Diospyros lotus)was conducted to explore the dwarfing genes related to the use of the‘Nantong-xiaofangshi’interstock.Hormone levels and water conductance were also measured in these two graft combinations.The results indicated that the levels of both IAA and GA were lower in‘Kanshu’that had been grafted onto the‘Nantong-xiaofangshi’interstock than in‘Kanshu’with no interstock;additionally,the water conductance was lower in grafts with interstocks than in grafts without interstocks.The expression of AUX/IAA and auxin-responsive GH3 genes was enhanced in scions grafted on the interstock and was negatively correlated with the IAA content and growth of scions.The expression of GA2ox,DELLA,and SPINDLY genes were also upregulated and associated with a decrease in the level of GA in scions grafted on the interstock.Since one of the GA2ox unigenes was annotated as DkGA2ox1 in Diospyros kaki,but was not functionally validated,a functional analysis was conducted in transgenic tobacco.Overexpression of DkGA2ox1 in transgenic plants resulted in a dwarf phenotype that could be recovered by the exogenous application of GA3.We conclude that the‘Nantong-xiaofangshi’interstock affects the water conductance and expression of genes related to the metabolism and transduction of IAA and GA in the grafted scion and thus regulates phytohormone levels,producing dwarfing.

关 键 词：METABOLISM SWEET DWARF 

分 类 号：R73[医药卫生—肿瘤]