Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development  被引量:6

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作  者:Danielle Costenaro da Silva Vítor da Silveira Falavigna Marianna Fasoli Vanessa Buffon Diogo Denardi Porto Georgios Joannis Pappas Jr Mario Pezzotti Giancarlo Pasquali Luís Fernando Revers 

机构地区:[1]Graduate Program in Cell and Molecular Biology,Center for Biotechnology,Universidade Federal do Rio Grande do Sul,Porto Alegre,91501-970,RS,Brazil [2]Dipartimento di Biotecnologie,Universitàdegli Studi di Verona,Verona,37134,Italy [3]Embrapa Uva e Vinho,Bento Gonçalves,95701-008,RS,Brazil [4]Embrapa Semiárido,Petrolina,56302-970,PE,Brazil [5]Department of Cell Biology,Universidade de Brasília,Brasília,70910-900,DF,Brazil [6]Syngenta Seeds Ltda,Uberlândia,MG,38407-049,Brazil [7]Modesto,CA 95354,USA

出  处:《Horticulture Research》2016年第1期124-133,共10页园艺研究(英文)

基  金:This work was supported by‘Coordenação de Aperfeiçoamento de Pessoal de Nível Superior’(CAPES,Brazil),‘Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq,Brazil,Proc.475440/2004-8,GP research fellowship 311361/2009-9),‘Fundação de AmparoàPesquisa do Estado do Rio Grande do Sul’(FAPERGS,Brazil,Proc.04/1184.6)and Embrapa(Brazil).

摘  要:The Dof(DNA-binding with one finger)protein family spans a group of plant transcription factors involved in the regulation of several functions,such as plant responses to stress,hormones and light,phytochrome signaling and seed germination.Here we describe the Dof-like gene family in grapevine(Vitis vinifera L.),which consists of 25 genes coding for Dof.An extensive in silico characterization of the VviDofL gene family was performed.Additionally,the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray(cv Corvina)and real-time PCR(cv Pinot Noir)analyses.The phylogenetic analysis comparing grapevine sequences with those of Arabidopsis,tomato,poplar and already described Dof genes in other species allowed us to identify several duplicated genes.The diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological roles.Furthermore,distinct expression patterns were identified between samples analyzed,corroborating such hypothesis.Our expression results indicate that several VviDofL genes perform their functional roles mainly during flower,berry and seed development,highlighting their importance for grapevine growth and production.The identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir.

关 键 词:INVOLVEMENT ORGANS assessed 

分 类 号:R73[医药卫生—肿瘤]

 

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