Single-Atom Nanozymes Linked Immunosorbent Assay for Sensitive Detection of Aβ1-40:A Biomarker of Alzheimer’s Disease  

作　　者：Zhaoyuan Lyu Shichao Ding Nan Zhang Yang Zhou Nan Cheng Maoyu Wang Mingjie Xu Zhenxing Feng Xiangheng Niu Yuan Cheng Chao Zhang Dan Du Yuehe Lin 

机构地区：[1]School of Mechanical and Materials Engineering,Washington State University,Pullman,WA 99164,USA [2]Institute of High Performance Computing,Institute of High Performance Computing,Singapore 138632 [3]School of Chemical,Biological,and Environmental Engineering,Oregon State University,Corvallis,OR 97331,USA [4]Irvine Materials Research Institute(IMRI),University of California,Irvine,CA 92697,USA [5]Department of Electrical and Computer Engineering,National University of Singapore,Engineering Drive 3,Singapore 117583

出　　处：《Research》2020年第1期777-787,共11页研究（英文）

基　　金：This work was supported by a start-up fund from Washington State University.

摘　　要：Single-atom nanozymes(SANs)possess unique features of maximum atomic utilization and present highly assembled enzyme-like structure and remarkable enzyme-like activity.By introducing SANs into immunoassay,limitations of ELISA such as low stability of horseradish peroxidase(HRP)can be well addressed,thereby improving the performance of the immunoassays.In this work,we have developed novel Fe-N-C single-atom nanozymes(Fe-N_(x)SANs)derived from Fe-doped polypyrrole(PPy)nanotube and substituted the enzymes in ELISA kit for enhancing the detection sensitivity of amyloid beta 1-40.Results indicate that the Fe-N_(x)SANs contain high density of single-atom active sites and comparable enzyme-like properties as HRP,owing to the maximized utilization of Fe atoms and their abundant active sites,which could mimic natural metalloproteases structures.Further designed SAN-linked immunosorbent assay(SAN-LISA)demonstrates the ultralow limit of detection(LOD)of 0.88 pg/mL,much more sensitive than that of commercial ELISA(9.98 pg/mL).The results confirm that the Fe-N_(x)SANs can serve as a satisfactory replacement of enzyme labels,which show great potential as an ultrasensitive colorimetric immunoassay.

关 键 词：ALZHEIMER thereby limitations 

分 类 号：R749.16[医药卫生—神经病学与精神病学]