植物乳杆菌X7021吸附重金属和降解亚硝酸盐能力的研究  

Study on the ability of Lactobacillus plantarum X7021 in the adsorption of heavy metals and degradation of nitrite

作　　者：张奕吉 杜磊[1] 谢静莉[1] ZHANG Yi-Ji;DU Lei;XIE Jing-Li(State Key Laboratory of Bioreactor Engineering,Department of Food Science and Engineering,School of Biotechnology,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,生物工程学院食品科学与工程系,上海200237

出　　处：《食品安全质量检测学报》2021年第12期4786-4792,共7页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(31801489)。

摘　　要：目的研究植物乳杆菌X7021吸附重金属与降解亚硝酸盐的能力。方法采用分光光度法检测Cu^(2+)和亚硝酸盐含量,采用石墨炉原子吸收分光光度法检测Cd^(2+)和Pb^(2+)含量。探讨pH、温度、吸附时间和离子浓度等因素对植物乳杆菌X7021吸附重金属能力的影响,以及亚硝酸盐浓度对其生长和降解能力的影响。结果植物乳杆菌X7021对重金属的吸附率和吸附量均受到吸附条件的影响。首先,吸附量随着金属离子浓度的增加而增加,而吸附率呈现出先快速下降后趋于平缓的趋势。其次,吸附率和吸附量均随吸附时间增加而增加,且植物乳杆菌X7021对Pb^(2+)的吸附量明显优于Cu^(2+)和Cd^(2+)。第三, Cu^(2+)、Cd^(2+)和Pb^(2+)的最大吸附率分别在pH=7、7和6;且温度为37℃时,Cu^(2+)和Cd^(2+)的吸附率和吸附量均达到最大。除此之外,植物乳杆菌X7021在24h内几乎完全降解100 mg/L NaNO2,且可耐受浓度高达700 mg/L的亚硝酸盐。结论植物乳杆菌X7021能够有效吸附Cu^(2+)、Cd^(2+)、Pd2+等重金属离子,且对亚硝酸盐有较强的降解和耐受能力,在发酵食品及益生菌领域具有潜在的应用前景。Objective To explore the abilities of Lactobacillus plantarum X7021 on adsorbing heavy metals and degrading nitrites. Methods Cu^(2+) and nitrites content was detected by spectrophotometry, and Cd^(2+) and Pb^(2+) content was detected by graphite furnace atomic absorption spectrophotometry. Furthermore, the effects of time, pH, temperature and heavy metal ions concentration on the adsorption ratio and capacity and effects of nitrite concentration on its growth and degradation ability were investigated. Results Both the adsorption ratio and capacity of L. plantarum X7021 on heavy metal ions were affected by many factors. Firstly, the adsorption capacity increased with the increase of the metal ions concentration, while the adsorption ratio showed a rapid decline firstly and then came to stable. Secondly, the adsorption ratio and capacity increased with increasing adsorption time, and the adsorption capacity of Pb^(2+) by Lactobacillus plantarum X7021 was significantly higher than Cu^(2+) and Cd^(2+). Thirdly, the maximum adsorption ratio of Cu^(2+), Cd^(2+) and Pb^(2+) were at pH 7, 7 and 6, respectively, and the adsorption ratio and capacity of Cu^(2+) and Cd^(2+) reached the maximum at 37 ℃. In addition, L. plantarum X7021 almost degraded 100 mg/L NaNO2 within 24 h completely, and could tolerate nitrite with a concentration up to 700 mg/L. Conclusion L. plantarum X7021 can effectively adsorb Cu^(2+), Cd^(2+) and Pd2+, and has a strong ability to degrade and tolerate nitrite, so it has a promising application in fermented food and probiotics.

关 键 词：植物乳杆菌X7021 重金属 吸附 亚硝酸盐 降解 

分 类 号：TS201.3[轻工技术与工程—食品科学]