‘白叶1号’返白过程中泛素化酶基因序列分析与表达  

Sequence Analysis and Expression of Ubiquitinase Genes in the Process of'Baiye1'(Camellia sinensis)Returning to White

作　　者：彭靖 李银花[1,2,3,4] 王坤波 金可[1,2] 李娟 Peng Jing;Li Yinhua;Wang Kunbo;Jin Ke;Li Juan(College of Horticulture,Hunan Agricultural University,Changsha,410128;Key Laboratory of Tea Science,Ministry of Education,College of Horticulture,Hunan Agricultural University,Changsha,410128;National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals,Changsha,410128;Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients,Changsha,410128)

机构地区：[1]湖南农业大学园艺学院,长沙410128 [2]湖南农业大学园艺学院,茶学教育部重点实验室,长沙410128 [3]国家植物功能成分利用工程技术研究中心,长沙410128 [4]湖南省植物功能成分利用协同创新中心,长沙410128

出　　处：《分子植物育种》2021年第14期4637-4648,共12页Molecular Plant Breeding

基　　金：国家自然科学基金(31500567,U19A2030);湖南省作物种质创新与资源利用重点实验室科学基金开放项目(16KFXM14)共同资助。

摘　　要：‘白叶1号’(Camellia sinensis cv.Baiye 1)是一种遇低温发生阶段性返白的特异茶树品种。泛素化酶系统由泛素活化酶E1、泛素结合酶E2、泛素连接酶E3组成,前期从‘白叶1号’阶段性返白过程叶片中分离了多个泛素化酶差异表达基因。本研究以‘白叶1号’返白前期、返白期和复绿期的鲜叶为原料,探讨了‘白叶1号’返白过程中主要生化成分含量与前期获得的五个泛素化酶基因表达模式的关系。返白期氨基酸、没食子酸、生物碱含量较高,儿茶素含量较低;5个基因在复绿期的表达量皆高于返白期。5个泛素化酶蛋白中都含有多个磷酸化位点,CsE3-2含有的磷酸化位点最多;CsE1-1、CsE1-2为细胞核内蛋白,CsE2定位在高尔基体膜上,CsE3-1定位在核膜上,参与诱导细胞凋亡,CsE3-2可能为内质网膜内在蛋白,参与内质网相关降解(ERAD)通路;CsE3-2蛋白的二级结构主要是α-螺旋,其他4个泛素化酶的二级结构主要是无规则卷曲。研究结果表明:(1)泛素化酶系统可能参与了‘白叶1号’返白过程中氨基酸含量上升的响应过程;(2)儿茶素含量在返白期的降低可能与叶绿体的解体有关;(3)5个泛素化酶参与了泛素蛋白酶体途径和泛素非蛋白酶体途径中的多个调控过程。本研究对于进一步认识‘白叶1号’阶段性返白过程中的分子调控机制和揭示茶树生长发育的精确调控过程有一定的意义。'Baiye1'(Camellia sinensis cv.Baiye1)is a special tea cultivar with the stage of turning back to white in the event of low temperature.Ubiquitinase system is composed of ubiquitin activating enzyme E1,ubiquitin binding enzyme E2 and ubiquitin ligase E3.In the previous studies,several differential expression genes of ubiquitin were isolated from the leaves of'Baiye1'during the stage of returning to white.In this study,the fresh leaves of'Baiye1'in the early stage of whiteness,the white stage and the green stage were used as experimental materials.The relationship between the content of main biochemical components and the expression patterns of five ubiquitinase genes was discussed.The contents of amino acid,gallic acid and alkaloid were higher and catechins were lower in the white stage.The expression level of the five genes in the green stage was higher than that in the white stage.There are many phosphorylation sites in all five ubiquitinase proteins.CsE3-2 contains the most phosphorylation sites.CsE1-1 and CsE1-2 are nuclear proteins.CsE2 is located on Golgi membrane,and CsE3-1 is located on the nuclear membrane,which is involved in inducing apoptosis.CsE3-2 may be an Endoplasmic reticulum intrinsic protein,which is involved in the endoplasmic reticulum associated degradation(ERAD)pathway.The secondary structure of CsE3-2 protein is mainlyα-helix,and the other four ubiquitinases are mainly irregular curl.The results showed that:(1)The ubiquitinase system may be involved in the response process of amino acid content rising in the process of'Baiye1'returning to white.(2)The decrease of catechin content in the white stage may be related to the disintegration of chloroplast.(3)Five ubiquitinases are involved in the regulation of ubiquitin proteasome pathway and ubiquitin non proteasome pathway.This study has a certain significance for further understanding the molecular regulation mechanism of'Baiye1'in the process of turning back to white and revealing the precise regulation process of tea growth and development.

关 键 词：‘白叶1号’ 泛素化酶基因 生化成分 序列分析 表达分析 

分 类 号：S571.1[农业科学—茶叶生产加工]